Susceptible Mouse Strains
Susceptible Mouse Strains - Related Videos
06:51
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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Cited by 53 •
2016
This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.
08:38
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
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Cited by 1 •
2012
Certain mouse strains are able to resist induction of experimental autoimmune encephalomyelitis (EAE) with myelin basic protein. Described here is a simple immunization protocol that reverses the unresponsiveness and induces paralytic disease in several typical EAE resistant mouse stains.
10:24
Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment
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Cited by 57 •
2015
This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences.
02:58
Measurement of Compressive Stress-Strain Response at Small-Strains
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2025
This protocol presents the configuration of a compression test device capable of precisely measuring the mechanical properties of microstructures in the small-strain region, along with a systematic method for compression testing using this device. The proposed device can be used to analyze various microstructures and the mechanical behavior of polymer-based materials.
03:18
Stimulation of the Intracardiac Nervous System to Assess Susceptibility to Arrhythmia
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2025
Source: Jungen, C., et al. Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. J. Vis. Exp. (2018).The video demonstrates an ex vivo technique for stimulating the intracardiac nervous system to assess the heart’s susceptibility to arrhythmia. Using a perfusion apparatus, an isolated mouse heart is perfused with an oxygenated buffer. The intracardiac nervous system is then stimulated at a high frequency to induce arrhythmias.
FAQs
Genetic background can alter several checkpoints in host defense. Differences in pathogen recognition may affect how innate immune cells detect infection, while variation in cytokine responses can shape the signaling that follows. Changes in antigen presentation or immune regulation can further influence adaptive responses. Together, these strain-dependent features help explain why the same pathogen may produce different levels of replication or disease severity.
Comparing susceptible and resistant strains can separate host contribution from pathogen behavior. If both strains encounter the same infectious challenge, differences in replication or disease severity point toward effects of genetic background and immune function rather than microbial exposure alone. This contrast helps investigators identify protective immune mechanisms and interpret susceptibility as a host-dependent experimental variable.
Cytokine responses matter because they connect early pathogen recognition with broader immune regulation. Strain-dependent differences in these signals can alter the balance between effective defense and the immune conditions associated with severe disease. Examining cytokine behavior alongside antigen presentation and adaptive responses therefore gives a more complete explanation of why infection outcomes vary between mouse strains.
Characterization requires measuring infection or disease outcomes in a defined genetic background and relating those outcomes to immune features. Investigators can assess pathogen replication, disease severity, pathogen-recognition responses, cytokine responses, antigen presentation, and immune regulation as connected variables. This approach avoids treating susceptibility as a single endpoint and supports clearer comparisons among strains.
These strains are especially useful when researchers need a model that makes infection or disease outcomes easier to observe. They support studies of host-pathogen interactions, protective immune mechanisms, vaccines, and antimicrobial treatments. Comparing responses across susceptible genetic backgrounds can reveal whether an intervention changes pathogen replication or disease severity in a host context that differs from resistant strains.
Genetic background must be treated as part of the experimental design, not merely as a mouse source. A result observed in a susceptible strain may reflect interactions among host immunity, pathogen replication, and disease severity. Comparing outcomes across backgrounds helps distinguish microbial effects from host factors and makes conclusions about vaccines, treatments, or protective mechanisms more precise.