Vesicular Stomatitis Virus
Vesicular Stomatitis Virus - Related Videos
01:45
Vesicular Tubular Clusters
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2023
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi. With the help of motor proteins such...
07:03
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
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Cited by 38 •
2010
We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
11:09
Relating Stomatal Conductance to Leaf Functional Traits
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Cited by 39 •
2015
Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
08:55
Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats
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Cited by 3 •
2016
Oncolytic virotherapies are under development as novel therapeutics for the treatment of hepatocellular carcinoma (HCC). Here we describe a method for locoregional therapy of HCC via hepatic arterial administration of oncolytic virus.
12:08
Ex Vivo Infection of Live Tissue with Oncolytic Viruses
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Cited by 22 •
2011
Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
FAQs
Vesicular Stomatitis Virus entry depends on its surface glycoprotein, which enables the enveloped virion to enter host cells. After entry, the viral RNA-dependent RNA polymerase acts in the cytoplasm, where it transcribes and replicates the negative-sense RNA genome. These linked steps provide defined processes for investigating infection and antiviral responses.
The matrix protein can suppress host gene expression, helping the virus redirect cellular activity toward rapid viral production. This activity also influences innate immune responses, including interferon signaling. Studying the matrix protein therefore connects viral replication with the host response and helps explain how VSV can both multiply efficiently and shape antiviral defenses.
Interferon signaling is a central part of the innate response affected during VSV infection. Because viral components, including the matrix protein, can shape this response, VSV allows researchers to examine how viral replication and antiviral defense interact. The system is consequently useful for analyzing mechanisms that influence early immune control of infection.
VSV provides a model in which viral entry, cytoplasmic genome activity, host gene-expression suppression, and interferon responses can be considered together. This makes it useful for studying viral immunology rather than examining replication in isolation. Investigators can use the system to connect specific viral functions with broader outcomes in host defense and pathogenesis.
VSV supports several translational research areas, including antiviral defense studies, vaccine design, viral-vector development, and oncolytic therapy. Its value comes from the ability to investigate viral functions while considering how those functions might be adapted for medical or experimental purposes. These applications extend VSV research from mechanism-focused immunology toward therapeutic and preventive strategies.
VSV causes vesicular disease primarily in livestock, giving the virus direct importance in veterinary infection research. At the same time, its use as a model for viral immunology allows studies of pathogenesis and host responses in a broader experimental context. This combination links animal disease investigation with research on antiviral defense, vaccines, and viral vectors.