Derivative Analysis

Derivative analysis is the study of how quantities change, using derivatives to describe instantaneous rates of change and the local behavior of functions. In calculus, a derivative is defined through the limit of a difference quotient as the change in the input approaches zero; its value gives the slope of a function’s tangent line at a point. By examining derivatives, students and researchers can identify increasing or decreasing behavior, locate extrema, analyze concavity, and construct accurate models of dynamic systems. These tools support applications in optimization, motion, economics, engineering, and the analysis of complex mathematical relationships.

Derivative Analysis - Related Videos

Research

JoVE Journal - Immunology and Infection

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

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Cited by 122 •

2017

This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

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Cited by 11 •

2013

We describe an analytical method to estimate the lifetime of glutamate at astrocytic membranes from electrophysiological recordings of glutamate transporter currents in astrocytes.

Research

JoVE Journal - Biology
Free Sample

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

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Cited by 42 •

2017

The extracellular matrix plays a major role in defining the microenvironment of cells and in modulating cell behavior and phenotype. We describe a rapid method for the isolation of cell-derived extracellular matrix, which can be adapted to different scales for microscopic, biochemical, proteomic, or functional studies.

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

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Cited by 39 •

2011

This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

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Cited by 275 •

2015

Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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