Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

The overall goal of this experiment is to assess the various markers expressed by macrophage subpopulations within the central nervous system or CNS. This method can help answer key questions in the neuroimmunology field, such as duperry macrophages as we try to CNS doing dizzies, and what are the phenotypes of CNS in macrophages. The main advantages of this technique are the isolation of a large number of highly purified macrophage sub-population, as well as a contification of macrophage sub-population cell marker.

Begin by using fine scissors to cut the ventral skin just below the xiphoid process to expose the thoracic cavity. Make an incision in the diaphragm, and cut the lateral aspects of the ribcage in a caudal to rostral direction to expose the heart. After identifying the left ventricle and the right atrium, insert a butterfly catheter with a 25 gauage needle into the left ventricle.