Embryonic Chick Heart
Embryonic Chick Heart - Related Videos
07:47
A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
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Cited by 12 •
2013
Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.
04:34
Obtaining Cerebellum Slices from an Embryonic Chick Brain
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2025
The video demonstrates the preparation of cerebellum slices. It shows a detailed procedure for isolating the cerebellum from a chick embryo. The slices are then prepared from the dissected cerebellum using a tissue chopper for further experimental analysis.
06:15
In Ovo Electroporation in Embryonic Chick Retina
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Cited by 10 •
2012
The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.
06:31
In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
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Cited by 13 •
2016
The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.
06:23
Immunostaining of Dissected Zebrafish Embryonic Heart
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Cited by 35 •
2012
A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.
FAQs
Cardiac precursor cells first merge into a primitive heart tube, establishing an early organized structure. That tube then begins rhythmic contractions, providing an observable functional milestone during development. Subsequent looping, chamber formation, and differentiation of cardiac tissues progressively reshape the organ and create the stages needed to study vertebrate cardiac morphogenesis.
Looping and chamber formation transform the initially simple cardiac tube into a more structurally organized heart. These changes occur after rhythmic activity begins and accompany progressive differentiation of cardiac tissues. Observing their sequence helps investigators connect changes in cardiac shape with the broader process of heart formation rather than examining contraction alone.
Disruptions caused by genetic or environmental factors can alter the normal progression of cardiac morphogenesis, including the sequence of looping, chamber formation, or tissue differentiation. Studying these effects helps identify cellular mechanisms that shape the cardiovascular system and provides developmental context for understanding how abnormal heart formation may contribute to congenital heart defects.
Researchers can take advantage of the chick embryo's readily accessible position and the rapid timing of cardiac development. Observation can follow the transition from precursor-cell merging to heart-tube contraction, looping, chamber formation, and tissue differentiation. This progression allows developmental events and responses to genetic or environmental disruption to be examined within an accessible model.
Blood flow studies add a functional dimension to observations of cardiac shape and tissue development. By examining blood flow alongside rhythmic contractions, looping, and chamber formation, investigators can relate cardiac activity to morphogenesis. This combined perspective supports analysis of how developmental structure and function change during formation of the cardiovascular system.
The model links visible developmental events with questions relevant to human cardiovascular formation. Its accessible embryo and rapidly occurring cardiac changes support investigation of morphogenesis, blood flow, and developmental disruption. Findings can therefore help clarify cellular mechanisms underlying abnormal heart formation and provide scientific context for studying congenital heart defects in medicine.