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Chapters
- 00:05Title
- 01:08Preparation of Slides and Humidified Chamber
- 01:50Dissection of Embryonic Heart
- 03:21Immunostaining and Imaging
- 05:00Representative Heart Imaging Results
- 05:57Conclusion
A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.
Tags
ImmunostainingDissectedZebrafishEmbryonic HeartIn Vivo ModelCardiac DevelopmentHeart DiseasesEmbryosTransparent EmbryosCardiac DefectsGene Expression ManipulationMorpholino TechnologyRNA InjectionForward Genetic ScreensWhole Mount ImmunostainingExpression PatternTargeted ProteinHigh Resolution ImagesCellular StructuresSubcellular StructuresPhysical Location Of The HeartPoor Penetration Of AntibodiesStaining ProcessMicroscope SlideImmEdge PenFluorescence Signals
