Graft Fixation Sutures
Graft Fixation Sutures - Related Videos
05:22
Iris Fixation via External Pentagram Suturing
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2022
The present protocol describes how a 10-0 polypropylene suture crosses the anterior surface of the iris, forming a pentagram to prevent both iris and pupil from moving toward the cornea. This can be combined with subsequent keratoplasty to cure bullous keratopathy with extensive anterior synechia of the iris.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
06:36
A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects
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Cited by 1 •
2021
In this paper, we present an in vitro and in situ protocol to repair a tendon gap of up to 1.5 cm by filling it with engineered collagen graft. This was performed by developing a modified suture technique to take the mechanical load until the graft matures into the host tissue.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
05:47
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
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Cited by 14 •
2015
This report provides a detailed description of transplanting murine thymi from different aged donor mice under the kidney capsule of immunodeficient mouse recipients. The goal of this approach is to model T cell development and thymic selection events in vivo.
FAQs
Their immediate contribution is mechanical: stitches create contact between the graft and surrounding tissue or bone, resisting displacement during the period before biological integration develops. Stability helps preserve the graft’s intended anatomical alignment, while healing gradually establishes biological incorporation. Thus, fixation bridges a temporary mechanical need and a longer-term tissue-healing process.
Suture material, needle design, placement pattern, and the strength of the tissues receiving the fixation all influence performance. These factors affect how effectively the construct maintains contact and how forces are managed at the graft interface. Selection therefore cannot be separated from the graft and recipient anatomy, because a technique that exceeds tissue strength may compromise fixation.
Fixation must limit movement without applying excessive tension to the graft or recipient tissues. Too little stability may allow loosening or migration, whereas excessive tension can contribute to complications and may disturb the intended alignment. The operative goal is balanced fixation: enough mechanical restraint to preserve position while supporting, rather than disrupting, biological incorporation.
The surgeon positions the graft in its intended anatomical location, passes the needle and suture through the graft, and anchors or secures the construct through surrounding tissue or bone. Placement pattern and tissue strength then become important determinants of stability. This sequence is adapted to the repair site and aims to maintain alignment throughout healing.
They support several reconstructive settings, including ligament reconstruction, tendon repair, skin grafting, and vascular or soft-tissue reconstruction. The shared purpose is to keep replacement or transplanted tissue positioned while healing proceeds, but the relevant tissue environment differs among these procedures. That variation is why material choice and placement technique must be considered for the specific repair.
Appropriate fixation can preserve alignment, limit graft displacement, and promote incorporation as healing develops. Conversely, loosening, migration, or excessive tension represent fixation-related concerns that may undermine the repair. These outcomes explain why surgeons treat suture selection and placement technique as clinically important components of reconstructive procedures rather than as interchangeable technical details.