Intracranial Transplantation
Intracranial Transplantation - Related Videos
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
11:29
A Modified Method for Heterotopic Mouse Heart Transplantion
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Cited by 5 •
2014
A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
08:47
Intracranial Injection of Adeno-associated Viral Vectors
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Cited by 56 •
2010
Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.
FAQs
Graft survival depends on successful vascularization, which supplies the implanted material with needed nutrients, and on the host response to the graft. Excessive immune rejection or inflammation can damage transplanted cells or tissues before they become established. Researchers therefore examine survival together with inflammatory responses to determine whether a graft remains viable long enough to contribute to repair.
Survival alone does not demonstrate therapeutic success. Transplanted cells or tissues must integrate functionally with host neural circuits, meaning they must participate appropriately in the surrounding networks. Studying this integration helps distinguish a graft that persists anatomically from one that can modify injured or diseased neural tissue in a meaningful way.
Neural stem cells, differentiated cells, and supportive biomaterials represent distinct transplantation strategies. Stem cells may provide a source for neural repair, differentiated cells may be selected for a more specific cellular role, and biomaterials may support the transplanted component. Comparing these options helps researchers investigate which approach best promotes graft survival, integration, and safety.
Stereotactic delivery provides the precision needed to place a graft within a selected intracranial location. Accurate placement matters because graft behavior and functional integration depend on the relationship between the transplant and nearby host tissue. This precision also enables researchers to study how location influences repair, circuit interaction, and the outcome of transplantation experiments.
Following precise graft placement, researchers evaluate whether the transplant survives, becomes vascularized, and remains compatible with the host tissue. They also assess immune rejection or inflammation and investigate functional integration with neural circuits. Long-term observation is important because early graft presence does not establish durable repair or reveal delayed safety concerns.
Medical research examines this approach in conditions including Parkinson’s disease, spinal injury, traumatic brain injury, and other neurological disorders. The specific objective may be to repair damaged neural tissue or modify diseased circuits. Studying these applications can clarify both the therapeutic potential of cell-based brain repair and the biological limits that constrain safe, lasting recovery.