Mesenchymal Markers
Mesenchymal Markers - Related Videos
10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
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Cited by 16 •
2013
A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.
13:26
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
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Cited by 15 •
2018
Traditional methods of assessing adipogenic differentiation are cheap and easy to use, but are not specific to changes in gene expression. We have developed an assay to quantify mesenchymal cell differentiation into mature adipocytes using a lineage specific marker. This assay has diverse applications across basic research and clinical medicine.
06:02
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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Cited by 16 •
2016
Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.
07:23
Electroporation of Craniofacial Mesenchyme
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2011
Craniofacial cartilages develop in close contact with other tissues and are difficult to manipulate in live animals. We are using electroporation to deliver molecular tools during growth of the craniofacial skeleton while bypassing early embryonic effects. This approach will allow us to efficiently test candidate molecules in vivo.
05:38
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
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Cited by 13 •
2017
Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.
FAQs
A panel of mesenchymal markers is more informative than a single antigen because expression can vary among cells from different tissue sources and under different culture conditions. Differentiation state also changes the profile. Evaluating several positive markers together therefore helps researchers characterize a population while reducing the risk that one variable marker will be treated as decisive.
Positive markers such as CD73, CD90, CD105, and vimentin provide evidence of mesenchymal characteristics, whereas negative markers help exclude hematopoietic or endothelial populations. This two-sided strategy is important because detecting a positive antigen alone may not establish that the analyzed cells belong to the intended population. Inclusion of exclusion markers improves the interpretive specificity of cellular characterization.
Marker expression should be interpreted in relation to cell state, not as a fixed identity label. Cells may show different profiles after changes in culture conditions or during differentiation, and tissue source can contribute additional variation. Consequently, mesenchymal markers are most useful when the experimental context is reported alongside the observed pattern, especially when comparing cell preparations.
Flow cytometry, immunostaining, and immunoblotting are distinct detection approaches listed for assessing these proteins and surface antigens. The important interpretive point is that the assay does not remove biological variability: whichever method is selected, researchers should evaluate marker combinations and account for tissue source, culture conditions, and differentiation state.
A practical workflow begins by selecting several commonly assessed positive markers, such as CD73, CD90, CD105, or vimentin, together with negative markers that exclude hematopoietic or endothelial cells. Researchers then choose flow cytometry, immunostaining, or immunoblotting for detection and interpret the resulting pattern in light of tissue source, culture conditions, and differentiation state.
In medicine, these markers support characterization of cells intended for tissue engineering and regenerative research, where confirming the cellular profile is important for interpreting experimental results. They also contribute to investigations of tumor progression and the tumor microenvironment. Their value extends beyond identification because marker patterns help researchers compare cell populations across experimental contexts.