Brdu Labeled Neurons
Brdu Labeled Neurons - Related Videos
12:46
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
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Cited by 6 •
2011
Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
09:21
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
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Cited by 60 •
2010
We demonstrate the use of the gene gun to introduce fluorescent dyes, such as DiI, into neurons in brain slices from rodents and non-human primates of different ages. In this particular case, we use adult mice (3-6 months old) and adult cynomologus monkeys (9-15 years old). This technique, originally described by the laboratory of Dr. Lichtman (Gan et al., 2000), is well suited for the study of dendritic branching and dendritic spine morphology and can be combined with traditional...
06:56
Measuring Exocytosis in Neurons Using FM Labeling
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Cited by 7 •
2006
The ability to measure the kinetics of vesicle release can help provide insight into some of the basics of neurotransmission. Here we used real-time imaging of vesicles labeled with the red fluorescent dye FM 4-64 to measure the rate of presynaptic vesicle release in hippocampal neuronal cultures.
04:35
Isolating Fluorescently Labeled Neurons from a Murine Brain
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2025
This video demonstrates a method to isolate fluorescently labeled neurons from a mouse brain.
FAQs
BrdU incorporation identifies cells that were synthesizing DNA during the S phase of the cell cycle. In neural tissue, this provides a time-linked marker for dividing neural precursor cells rather than a general label for all cells. Researchers can therefore use the presence of BrdU to examine when new cellular production occurred and relate it to later neuronal development.
BrdU labeling alone indicates DNA synthesis, but it does not establish that a labeled cell became a neuron. Researchers assess neuronal identity with cell-specific markers, allowing them to distinguish newly generated neurons from other BrdU-positive cells. This combined analysis strengthens conclusions about neurogenesis and helps characterize the cellular outcome of precursor-cell division.
The distinction depends on examining BrdU labeling together with markers that identify particular cell types. A BrdU-positive cell may represent a neural precursor or another DNA-synthesizing cell, so neuronal marker expression provides the necessary identity information. This approach prevents researchers from interpreting all DNA-synthesizing cells as neurons and supports more precise analysis of neuronal development.
After BrdU has marked DNA synthesis, researchers detect the label using immunohistochemistry, a technique that visualizes specific molecular targets in tissue sections. They then assess neuronal identity with cell-specific markers and examine the labeled cells in their anatomical context. This workflow connects the original labeling event with cellular identity and developmental observations.
Analysis of labeled cells can follow several stages of neuronal development. Their presence indicates newly produced cells, while their locations can provide information about movement through brain tissue, and their neuronal marker profile can support assessment of maturation. Together, these observations help researchers study how new neurons emerge and develop rather than measuring proliferation alone.
The method is useful for investigating adult neurogenesis, neural development, and responses to neural injury. It also supports experiments examining how environmental or other experimental treatments affect cell proliferation. By combining BrdU detection with neuronal markers, researchers can evaluate whether changes in DNA-synthesizing cells are associated with the production and development of new neurons.