Dentate Granule Cells
Dentate Granule Cells - Related Videos
07:00
In Vivo Calcium Imaging of Granule Cells in the Dentate Gyrus of Hippocampus in Mice
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Cited by 1 •
2024
The dentate gyrus of the hippocampus carries out essential and distinct functions in learning and memory. This protocol describes a set of robust and efficient procedures for in vivo calcium imaging of granule cells in the dentate gyrus in freely moving mice.
10:55
Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice
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Cited by 6 •
2015
We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.
16:01
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
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Cited by 9 •
2015
The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's morphology.
04:43
A Technique to Isolate Nuclei from Non-neuronal Cells in the Hippocampal Dentate Gyrus
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2025
The video demonstrates a method for isolating intact nuclei from glial and stem cells in the mouse hippocampal dentate gyrus (DG). The DG tissue is mechanically dissociated in a cold homogenization buffer to release the nuclei. Tissue debris and other released organelles are discarded, and the nuclei are stained with a DNA stain and a fluorophore-conjugated antibody specific for the neuronal nuclear antigen (NeuN). Fluorescence-activated nuclei sorting is then employed to isolate the...
09:10
Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
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Cited by 8 •
2015
During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.
FAQs
Sparse activation in dentate granule cells helps the dentate gyrus avoid representing every incoming cortical signal in the same way. This relative selectivity means that overlapping experiences can recruit different subsets of cells, supporting pattern separation. The resulting distinction helps the hippocampal circuit maintain separate memory traces.
Their extensive mossy fiber projections connect dentate granule cells with CA3 pyramidal neurons. This pathway links signal transformation in the dentate gyrus to downstream hippocampal processing. Because CA3 receives the resulting granule-cell output, differences in which cells become active can influence whether related experiences remain represented as separate memory traces.
Dentate granule cells transform incoming cortical signals before those signals reach the hippocampal circuit. In this sequence, sparse activity changes how similar inputs are represented rather than passing them forward as indistinguishable patterns. This preprocessing supports distinct representations that can contribute to separating related experiences in memory.
In many mammals, neural stem cells can generate new dentate granule cells during adulthood. This provides a way to examine how newly arising neurons participate in neurogenesis, synaptic plasticity, and circuit maturation. The model is especially useful for asking how cellular addition may relate to changing hippocampal function across the lifespan.
Their sparse activity and connections with CA3 pyramidal neurons provide a cellular and circuit framework for examining how similar experiences acquire distinct memory representations. Studying these cells therefore connects neuronal activity patterns with pattern separation, while adult neurogenesis adds a developmental dimension to investigations of learning, memory, and synaptic plasticity.
Research on dentate granule cells contributes to studies of stress, aging, epilepsy, and hippocampal disorders. These cells are informative because their activity patterns, mossy fiber projections, and potential for adult generation connect circuit organization with plasticity and maturation. Findings can therefore place disease-related changes within broader hippocampal mechanisms.