Nerve Stump Dissection
Nerve Stump Dissection - Related Videos
17:05
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction
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Cited by 13 •
2017
Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.
08:05
Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap
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Cited by 10 •
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This article describes a technique to insert a hollow conduit between the spinal cord stumps after complete transection and fill with Schwann cells (SCs) and injectable basement membrane matrix in order to bridge and promote axon regeneration across the gap.
11:02
Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
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Cited by 10 •
2011
Optic Nerve transection is a widely used model of adult CNS injury. This model is ideal for performing a number of experimental manipulations that target the retina globally or directly target the injured neuronal population of retinal ganglion cells.
09:48
Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats
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Cited by 9 •
2020
The major pelvic ganglia contain parasympathetic and sympathetic neurons that innervate pelvic organs. Here we describe a dissection method and provide schematics for identification of these ganglia and their associated nerves. These methods can be applied to experimental manipulation of these ganglia in vivo or removal post-mortem for further study.
09:05
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
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Cited by 14 •
2013
Although the peripheral nervous system (PNS) is capable of significant repair after injury, little is known about the cellular and molecular mechanisms that govern this phenomenon. Using live, transgenic zebrafish and a reproducible nerve transection assay, we can study dynamic glial cell behaviors during nerve degeneration and regeneration.
FAQs
Comparing the proximal and distal stumps links structural observations to their different biological states. The proximal stump remains connected to the cell body, whereas the distal stump contains degenerating fibers. This distinction helps investigators examine injury responses alongside regeneration-related changes, including axonal sprouting, and provides a basis for interpreting how nerve damage affects different stump regions.
Careful removal of surrounding connective tissue is essential because the examination depends on exposing the severed nerve end rather than confusing it with adjacent tissue. Selective exposure allows the proximal and distal regions to be isolated and compared. Preserving the nerve itself supports assessment of degenerating fibers, regenerative responses, and changes associated with neuroma formation.
The distal stump is especially relevant to Wallerian degeneration because it contains fibers undergoing degeneration after severing. Examination can then be related to axonal sprouting as part of the regenerative response and to neuroma formation at the injury site. Considering these findings together helps distinguish degeneration, attempted regrowth, and changes associated with repair after nerve injury.
The procedure proceeds by exposing the severed nerve, carefully removing surrounding connective tissue, isolating the nerve without damaging it, and examining the proximal and distal stumps separately. This sequence preserves the anatomical distinction needed for interpretation. The resulting observations can relate stump features to injury responses, degenerating fibers, regenerative changes, and findings associated with neuroma formation.
Researchers use this approach when they need tissue-level evidence about injury responses or peripheral nerve regeneration. Examination of the stumps can help evaluate whether an experimental treatment influences regenerative responses, axonal sprouting, or neuroma formation. These observations support assessment of treatments designed to restore peripheral nerve function and help relate experimental findings to the original injury.
In neuroscience, the technique connects microsurgical tissue examination with broader questions about peripheral nerve repair. Observations from the stumps can inform analysis of injury mechanisms, regenerative responses, and neuroma formation. When used to assess an experimental treatment, the dissection supports anatomical evaluation of whether the intervention may contribute to restoring peripheral nerve function.