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Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
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Chapters
- 00:05Title
- 01:18Preparation and Mounting of Zebrafish Embryos
- 03:10Laser Calibration and Testing
- 04:35Nerve Transection Using Laser Ablation and Time-lapse Confocal Imaging of Glial Cell Behaviors
- 06:39Representative Laser Ablation Results
- 08:39Conclusion
Although the peripheral nervous system (PNS) is capable of significant repair after injury, little is known about the cellular and molecular mechanisms that govern this phenomenon. Using live, transgenic zebrafish and a reproducible nerve transection assay, we can study dynamic glial cell behaviors during nerve degeneration and regeneration.
