Neurolucida 360
Neurolucida 360 - Related Videos
07:38
Circumferential Transforaminal Endoscopic Lumbar Foraminotomy for 360° Exiting Nerve Root Decompression in Patients with Lumbar Foraminal Stenosis
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2026
This protocol describes circumferential transforaminal endoscopic lumbar foraminotomy for 360° decompression of the exiting nerve root in patients with lumbar foraminal stenosis using a step-by-step, landmark-based approach to achieve adequate bony and soft-tissue decompression.
02:38
Local Heat Probe Assay: A Method to Assess Nociception of High Temperatures in Drosophila Larvae
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2023
Drosophila larvae have dedicated sensory neurons–nociceptors–whose response to potentially harmful thermal stimuli triggers aversive–or so-called "nocifensive"–behaviors in the animal. This video describes a method to assess thermal nociception by recording larval behavior in response to local application of a heat probe onto the cuticle. This method is, therefore, called the local heat probe assay.
04:51
Isolation and Culture of Primary Neurons and Glia from a Rat Urinary Bladder
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2025
This video demonstrates a technique for generating a primary culture of neurons and glia from a rat's urinary bladder. It outlines the steps involved in tissue preparation, enzymatic digestion to obtain single cells, and culturing the cells in a neuron-specific medium to establish a primary neuronal and glial culture.
03:15
Photoacoustic Monitoring of Dye Movement in Cortical Blood Vessels
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2025
Source: Kalva, S. K., et al., Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature. J. Vis. Exp. (2019)This video demonstrates the procedure for using photoacoustic imaging to observe changes in the cortical vessels of an anesthetized rat. The process includes baseline imaging, dye injection, and analysis of dye absorption and clearance during wash-in and wash-out.
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
FAQs
Neurolucida 360 follows neuronal structures through successive images in a microscopy stack, maintaining their three-dimensional continuity rather than treating each image as an isolated view. Image-guided tracing can be automated or directed by the user, and the traced paths are assembled into digital models. These models provide a basis for examining neuronal morphology quantitatively.
These tracing approaches provide different levels of guidance during reconstruction. Image guidance links the tracing process to structures visible in the microscopy data, automation supports efficient following of neuronal features, and user direction allows the researcher to control the reconstruction. Together, they help represent complex cellular structures while retaining a direct connection to the underlying image stack.
The software can trace somata, dendrites, axons, and spines, allowing researchers to examine several dimensions of neuronal organization. Somata and processes contribute to cellular morphology, while dendrites, axons, and spines also help describe structural relationships relevant to synaptic organization and connectivity. Analyzing these components together supports a more complete representation of neuronal architecture.
Digital models convert detailed microscopy observations into standardized quantitative representations. Researchers can use those representations to measure and compare neuronal features across individual cells, brain regions, and studies. Standardization is important because it supports reproducible comparisons, making it easier to evaluate morphological differences associated with development, disease, or experimental manipulation.
A typical workflow begins with a microscopy image stack containing the neuronal structures of interest. The researcher then uses image-guided, automated, or user-directed tracing to follow structures across the stack, including somata, dendrites, axons, or spines. The resulting reconstruction becomes a digital model that can be measured for quantitative morphological analysis.
Neurolucida 360 is useful when researchers need to relate neuronal shape and structure to biological conditions. Reconstructions can support studies of cellular morphology, synaptic structure, connectivity, and circuit organization, including comparisons involving development, disease, or experimental manipulation. Its quantitative models help investigators assess how neuronal architecture changes across cells, regions, or study conditions.