Sirna Transfection
Sirna Transfection - Related Videos
08:21
Time-lapse Imaging of Mitosis After siRNA Transfection
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Cited by 8 •
2010
Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.
09:57
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
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Cited by 3 •
2016
This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.
09:14
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
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Cited by 4 •
2012
The article details the protocol for site-specific transfection of scrambled sequence of siRNA in an adherent mammalian cell culture using a microelectrode array (MEA).
04:34
siRNA Reverse Transfection-Based Gene Silencing In Vitro: A Procedure to Deliver Small Interfering RNA in Cultured Cells to Inactivate Target Gene Expression
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2025
In this video, we describe a procedure to perform reverse transfection of siRNA into adipocytes to modulate the expression of a target protein.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
FAQs
After entering the cell, siRNA becomes associated with the RNA-induced silencing complex, or RISC. The complex uses the siRNA sequence as a recognition guide, allowing it to identify messenger RNA with complementary sequence information. Matching transcripts are then degraded, reducing the amount of message available for protein production and linking sequence choice to gene-specific effects.
The silencing produced by siRNA transfection is temporary rather than a permanent change to the gene. This allows researchers to reduce expression during a defined experimental period and then examine the resulting cellular effects. In neuroscience, that reversibility supports investigations of gene function without requiring a permanently altered neuronal, glial, or neural culture system.
Sequence complementarity determines which messenger RNA the RNA-induced silencing complex can recognize. An siRNA sequence directed toward a selected transcript therefore connects the molecular intervention to a particular gene. The resulting reduction in that transcript, followed by reduced protein synthesis, provides a basis for relating gene-specific changes to signaling, synaptic, developmental, or disease-associated cellular effects.
A typical experiment focuses on the consequences of introducing synthetic siRNA into a selected cell system and then assessing reduced expression of the intended gene. Researchers can relate that molecular reduction to changes in cellular behavior or function. The approach is therefore organized around connecting gene suppression with measurable effects in neurons, glial cells, or neural cultures.
In neuroscience, this method is useful when investigators need to test how a particular gene contributes to signaling pathways, synaptic function, neurodevelopment, or disease mechanisms. Applying gene-specific suppression to neurons, glial cells, or neural cultures can reveal whether changing that gene alters a relevant cellular process, helping establish functional relationships rather than simple expression associations.
Reduced gene expression can help researchers determine whether the targeted gene is functionally connected to an observed cellular outcome. When suppression is associated with changes in neural signaling, synaptic activity, development, or disease-related behavior, the result supports target validation. These findings can also inform investigation of potential molecular interventions, although the method itself produces temporary silencing.