Beijing CytoNiche Biotechnology Co. Ltd. 1 article published in JoVE Bioengineering Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers Yuyang Chen*1, Huanye Xu*2, Yuanyuan Zhang*2, Leilei Guo2, Miaomiao Lan2, Yanping Yang2, Wei Liu2, Xiaojun Yan2, Yanan Du1 1Department of Biomedical Engineering, School of Medicine, Tsinghua University, 2Beijing CytoNiche Biotechnology Co. Ltd. Here, we present a protocol to achieve the large-scale manufacturing of adherent cells through a fully closed system based on GMP-grade dissolvable microcarriers. The cultivation of human mesenchymal stem cells, HEK293T cells, and Vero cells was validated and met both quantity demands and quality criteria for the cell and gene therapy industry.