Hannover Medical School View Institution's Website 11 articles published in JoVE Medicine Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model Oliver Beetz*1, Felix Oldhafer*1, Clara A. Weigle1, Sebastian Cammann1, Daphne DeTemple1, Lion Sieg2, Hendrik Eismann2, Thomas Palmaers2, Florian W. R. Vondran1 1Department of General, Visceral and Transplant Surgery, Hannover Medical School, 2Department of Anaesthesiology and Intensive Care Medicine, Hannover Medical School In this protocol, a model of porcine orthotopic liver transplantation after static cold storage of donor organs for 20 h without the use of a veno-venous bypass during engraftment is described. The approach uses a simplified surgical technique with minimization of the anhepatic phase and sophisticated volume and vasopressor management. Immunology and Infection A Modified Surgical Technique for Kidney Transplantation in Mice Decheng Yin*1,2, Jian Fu*1,3, Rongjun Chen4, Nelli Shushakova5, Ida Allabauer1, Xin-Yi Wei1, Mario Schiffer6, Diana Dudziak7, Song Rong*5, André Hoerning*1 1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, 5Department of Nephrology, Hannover Medical School, 6Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 7Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure. Medicine Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model Decheng Yin*1,2, Jian Fu*1,3, Ida Allabauer1, Oliver Witzke4, Song Rong5, André Hoerning1 1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, 5Department of Nephrology, Hannover Medical School A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated. Medicine Implantation of Combined Telemetric ECG and Blood Pressure Transmitters to Determine Spontaneous Baroreflex Sensitivity in Conscious Mice René D. Rötzer1, Verena F. Brox1, Konstantin Hennis1, Stefan B. Thalhammer1, Martin Biel1,2, Christian Wahl-Schott3, Stefanie Fenske1,2 1Center for Integrated Protein Science (CIPS-M) and Center for Drug Research, Department of Pharmacy, Ludwig-Maximilians-Universität München, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, 3Hannover Medical School, Institute for Neurophysiology The baroreflex is a heart-rate regulation mechanism by the autonomic nervous system in response to blood-pressure changes. We describe a surgical technique to implant telemetry transmitters for continuous and simultaneous measurement of electrocardiogram and blood pressure in mice. This can determine spontaneous baroreflex sensitivity, an important prognostic marker for cardiovascular disease. Immunology and Infection An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats Clara A. Weigle1, Thorsten Lieke1, Florian W. R. Vondran1, Kai Timrott1, Jürgen Klempnauer1, Oliver Beetz1 1Regenerative Medicine and Experimental Surgery, Department of General, Visceral and Transplant Surgery, Hannover Medical School We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats. Medicine Veno-Venous Extracorporeal Membrane Oxygenation in a Mouse Nodir Madrahimov1, Abdurasul Khalikov1, Erin C. Boyle1, Ruslan Natanov1, Ann-Kathrin Knoefel1, Thierry Siemeni1, Klaus Hoeffler1, Axel Haverich1, Ulrich Maus*2, Christian Kuehn*1 1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Pneumology, Hannover Medical School Here we present a protocol describing the technique of veno-venous extracorporeal membrane oxygenation (ECMO) in a non-intubated, spontaneously breathing mouse. This murine model of ECMO can be effectively implemented in experimental studies of acute and end-stage lung diseases. Medicine Cardiopulmonary Bypass in a Mouse Model: A Novel Approach Nodir Madrahimov1, Ruslan Natanov1, Erin C. Boyle1, Tobias Goecke1, Ann-Kathrin Knöfel1, Valentyna Irkha1, Anna Solovieva2, Klaus Höffler1, Ulrich Maus3, Christian Kühn1, Issam Ismail1, Gregor Warnecke1, Malakh-Lal Shrestha1, Serghei Cebotari*1, Axel Haverich*1 1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, 3Department of Pneumology, Hannover Medical School This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage. Neuroscience Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis Alexander Brinkmann*1,2, Camille Okom*1,2, Eugen Kludt1,2,4, Detlev Schild1,2,3 1Institute of Neurophysiology and Cellular Biophysics, Georg-August-Universität Göttingen, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Georg-August-Universität Göttingen, 3DFG Excellence Cluster 171, Georg-August-Universität Göttingen, 4German Hearing Center Hannover Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose. Developmental Biology A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells Claudia Davenport1, Ulf Diekmann1, Ortwin Naujok1 1Institute of Clinical Biochemistry, Hannover Medical School Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations. Developmental Biology Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis Verónica A. Lombardo*1,2,3, Cécile Otten*1,2, Salim Abdelilah-Seyfried1,2,3 1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA. Medicine Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens Dorothée Sturm1,2, Lorella Marselli3, Florian Ehehalt1,2, Daniela Richter1, Marius Distler2, Stephan Kersting1,2, Robert Grützmann2, Krister Bokvist4, Philippe Froguel5, Robin Liechti6, Anne Jörns7, Paolo Meda8, Gustavo Bruno Baretton9, Hans-Detlev Saeger2, Anke M. Schulte10, Piero Marchetti3, Michele Solimena1 1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.