CelVivo ApS 1 article published in JoVE Biochemistry Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures Stephanie Stransky1, Dejauwne Young1, Karoline Mikkelsen2,3, Annemette Præstegaard Thulesen2, Helle Sedighi Frandsen3, Simone Sidoli1 1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.