Center for Animal Health Research, INIA-CISA 1 article published in JoVE Immunology and Infection A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection Kevin Chiem1, Javier Rangel-Moreno2, Aitor Nogales1,3, Luis Martinez-Sobrido1 1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, 3Center for Animal Health Research, INIA-CISA Influenza A viruses (IAVs) are contagious respiratory pathogens that cause annual epidemics and occasional pandemics. Here, we describe a protocol to track viral infections in vivo using a novel recombinant luciferase and fluorescence-expressing bi-reporter IAV (BIRFLU). This approach provides researchers with an excellent tool to study IAV in vivo.