Trefoil Therapeutics, Inc. 1 article published in JoVE Medicine A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1 Sarah Pizzuto1, Grace Duffey1, Jessica Weant1, David Eveleth1 1Trefoil Therapeutics, Inc. Descemet's Stripping Only is an experimental procedure wherein patients with central corneal guttae resulting from Fuchs Endothelial Corneal Dystrophy have Descemet's membrane stripped for peripheral cells to regenerate the endothelial layer. We present novel methodology simulating DSO in dystrophic human corneas ex vivo with accelerated healing stimulated by eFGF1 (NM141).