Universidad de Chile 1 article published in JoVE Neuroscience Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors Andrés González-Gutiérrez1, Jorge Gaete1, Andrés Esparza1, Jorge Toledo2, Andrés Köhler-Solis1, Jimena Sierralta1,3 1Biomedical Neuroscience Institute, Universidad de Chile, 2Advanced Scientific Equipment Network (REDECA), Faculty of Medicine, Universidad de Chile, 3Department of Neuroscience, Faculty of Medicine, Universidad de Chile Here we present a protocol to visualize the transport of monocarboxylates, glucose, and ATP in glial cells and neurons using genetically encoded Förster resonance energy transfer-based sensors in an ex-vivo Drosophila larval brain preparation.
