Optical Metabolic Imaging
Optical Metabolic Imaging - Related Videos
11:39
Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants
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Cited by 49 •
2013
We combined frequency-domain near-infrared spectroscopy measures of cerebral hemoglobin oxygenation with diffuse correlation spectroscopy measures of cerebral blood flow index to estimate an index of oxygen metabolism. We tested the utility of this measure as a bedside screening tool to evaluate the health and development of the newborn brain.
04:47
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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2025
Utilizing a multimodal platform combining label-free optical imaging modalities, we have developed a protocol for visualizing and quantifying cellular dynamics and metabolism. Through imaging via multiphoton fluorescence, second harmonic generation, and stimulated Raman scattering microscopy, we can generate a holistic overview of the cellular and molecular environment.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
07:46
Optical Materialography Part 2: Image Analysis
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2023
Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA The imaging of microscopic structures of solid materials, and the analysis of the structural components imaged, is known as materialography. Often, we would like to quantify the internal three-dimensional microstructure of a material using only the structural features evidenced by an exposed two-dimensional surface. While X-ray based tomographical methods can reveal buried...
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
FAQs
The main signals come from the cofactors NAD(P)H and flavin adenine dinucleotide, or FAD. Because these molecules participate in cellular energy-related processes, their endogenous fluorescence provides a biochemical readout without adding labels. Examining both cofactors helps researchers relate optical measurements to cellular metabolism and changes in metabolic state.
Signal intensity indicates the amount of detected fluorescence, while fluorescence lifetime measures how long the signal persists after excitation. Relative NAD(P)H and FAD signals provide an additional comparison between metabolic cofactors. Together, these measurements can reveal shifts in redox balance and energy-producing pathways more informatively than relying on a single optical value.
Using endogenous fluorescence avoids introducing external labels that could interfere with the sample or complicate repeated observation. The resulting measurements can be collected noninvasively while cells or tissues remain living. This supports real-time phenotyping, meaning characterization of functional cellular behavior, and allows metabolic changes to be followed without disrupting the specimen.
The workflow centers on collecting endogenous fluorescence from living cells or tissues and analyzing signal intensity, fluorescence lifetime, and relative cofactor signals. These measurements are then interpreted in relation to redox balance, energy-producing pathways, or overall metabolic state. Quantitative analysis converts the optical observations into measurable indicators of cellular function.
In bioengineering, the technique can help evaluate whether cells within engineered tissues remain functionally active and how their metabolism changes over time. Because measurements do not require sample disruption, the same living construct can be monitored during development. This provides metabolic information alongside tissue development, supporting noninvasive assessment of engineered systems.
Researchers can compare endogenous fluorescence measurements before and after exposing cells or tissues to environmental or therapeutic conditions. Changes in cofactor intensity, lifetime, or relative signals may indicate altered redox balance or energy-producing activity. The approach therefore enables quantitative monitoring of metabolic responses while preserving the living sample for continued observation.