Aflatoxin B1
Aflatoxin B1 - Related Videos
09:44
RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
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Cited by 47 •
2015
We demonstrate a method for the analysis of aflatoxins and transgene expression in peanut seeds that contain RNA-interference signals for silencing aflatoxin-synthesis genes in the fungus Aspergillus flavus. RNAi-mediated control of mycotoxins in plants has not been reported previously.
10:24
Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
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2024
We demonstrate a medium-throughput method for quantification of aflatoxins and stilbenoid phytoalexins in single peanut seeds using ultra performance liquid chromatography. This method was specifically developed for the analyses of wild Arachis species challenged by the aflatoxigenic Aspergillus species.
09:21
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
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Cited by 11 •
2019
Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.
08:01
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
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Cited by 11 •
2016
A new electron paramagnetic resonance (EPR) method, rapid scan EPR (RS-EPR), is demonstrated for 2D spectral spatial imaging which is superior to the traditional continuous wave (CW) technique and opens new venues for in vivo imaging. Results are demonstrated at 250 MHz, but the technique is applicable at any frequency.
10:01
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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Cited by 39 •
2012
The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.
FAQs
Cytochrome P450 enzymes in the liver metabolically activate Aflatoxin B1 by converting it into a reactive epoxide. This transformation is central to toxicity because the original compound becomes a chemically reactive intermediate capable of interacting with cellular molecules. Studying this step helps explain why metabolism is a key focus in biological investigations of exposure.
When the reactive epoxide binds cellular DNA, it can produce mutations; binding to proteins can also disrupt cellular function. These molecular interactions connect AFB1 exposure with downstream biological injury rather than representing simple presence of the compound alone. Examining DNA and protein binding therefore helps researchers interpret molecular toxicity and its possible consequences.
Oxidative stress is an important part of the cellular response associated with AFB1 toxicity. Alongside DNA and protein interactions, it can contribute to impaired cell function, giving researchers another mechanism to investigate. Considering this stress helps place molecular damage within a broader biological picture of how exposure affects cells.
Biomarkers of exposure help connect contact with Aflatoxin B1 to biological investigation, even when the compound itself is not the only information of interest. Research on these markers supports exposure assessment and can complement studies of metabolism and molecular toxicity. This makes biomarkers useful in evaluating potential risks involving contaminated crops or animal feed.
Crop monitoring is a practical application of AFB1 research because crops can contribute to contamination of food and animal feed systems. Monitoring findings support risk assessment, while related prevention strategies aim to reduce contamination. Together, these activities help guide efforts to limit exposure and protect human and animal health.
Detoxification research examines mechanisms through which the harmful effects associated with AFB1 might be reduced. In biology, this work complements studies of metabolism, biomarkers, and molecular toxicity rather than replacing them. Its broader value lies in informing strategies to reduce contamination, support disease prevention, and protect human and animal health.