Blastocyst Stage
Blastocyst Stage - Related Videos
10:59
Human Blastocyst Biopsy and Vitrification
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Cited by 36 •
2019
Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Cited by 7 •
2010
In this video, we demonstrate the isolation of mouse blastocysts and the derivation of trophoblast stem cells from blastocysts. We also describe conditions for maintenance of the stem cell property as well as induction of differentiation in culture.
12:09
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Cited by 6 •
2022
A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
09:35
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
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Cited by 13 •
2017
MicroSecure Vitrification was developed as a non-commercial, aseptic closed vitrification device system that is compliant with FDA good manufacturing and tissue-handling practices. Due to the withdrawal of hydrophobic plugged embryo straws from the industry, the vitrification procedure was modified to include an inner seal before the standard internal cotton plug.
01:22
Stages of Sleep
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2024
Sleep progresses through distinct stages, each characterized by specific brain wave patterns and physiological responses ranging from wakefulness to stages of non-rapid eye movement, known as non-REM, to rapid eye movement, referred to as REM. Understanding these stages helps in recognizing how sleep supports various bodily and cognitive functions. Before sleep begins, in wakefulness, the brain exhibits primarily beta waves, which are high in frequency and low in amplitude, indicating alertness...
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Compaction changes the physical organization of the cells produced by cleavage divisions, bringing them into a more coordinated arrangement. Fluid accumulation then creates the blastocoel cavity and helps separate the outer and inner cell populations. Together, these processes transform an initially divided embryo into a structured system capable of continuing development and preparing for implantation.
The trophoblast and inner cell mass represent distinct developmental populations with different contributions. Trophoblast cells contribute to placental tissues, whereas cells of the inner cell mass give rise to the embryo proper. Their separation during blastocyst development provides an early example of lineage specification, in which cell groups acquire different developmental roles.
Cleavage divisions first increase cell number after fertilization. Subsequent compaction reorganizes those cells, while fluid accumulation produces the blastocoel cavity. The resulting arrangement establishes an outer trophoblast layer and an inner cell mass. This sequence links early cell division with structural reorganization, lineage separation, and preparation for uterine implantation.
Studying this stage helps investigators examine how an early embryo becomes prepared for implantation in the uterus and how its cell populations become organized. Disruptions in compaction, cavity formation, or lineage organization may help explain developmental failure. Consequently, blastocyst research connects visible early embryonic changes with questions about successful continuation of development.
In assisted reproductive technologies, the blastocyst stage provides a developmental point for studying embryos maintained in culture. Observing formation of the cavity, outer trophoblast, and inner cell mass can support investigation of how embryos progress before implantation. Embryo culture research therefore uses this stage to examine developmental progression in a controlled setting.
The inner cell mass is central to research on pluripotent stem cells because it gives rise to the embryo proper and reflects early developmental potential. Studying this population alongside trophoblast formation helps researchers investigate how lineages become specified. The blastocyst stage therefore provides biological context for understanding early cell potency and developmental decisions.