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JoVE Journal
Developmental Biology

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Human Blastocyst Biopsy and Vitrification

Human Blastocyst Biopsy and Vitrification

Article DOI: 10.3791/59625-v
July 26th, 2019


Summary July 26th, 2019

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.


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