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Human Blastocyst Biopsy and Vitrification
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Developmental Biology
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JoVE Journal Developmental Biology
Human Blastocyst Biopsy and Vitrification

Human Blastocyst Biopsy and Vitrification

DOI:
10.3791/59625-v

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10:59 min

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July 26, 2019

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, , , ,
1G.EN.E.R.A. Centers for Reproductive Medicine, 2DAHFMO, Unit of Histology and Medical Embryology,Sapienza University of Rome

Chapters

  • 00:04Title
  • 01:25Trophectoderm Biopsy
  • 04:50Tubing
  • 06:17Blastocyst Vitrification
  • 08:20Results: Representative Human Blastocyst Vitrification Quality Analyses
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

Tags

Keywords: Blastocyst BiopsyVitrificationIVFPreimplantation Genetic TestingTrophectoderm BiopsyZona PellucidaLaser ObjectiveBiopsy PipetteHEPES-buffered MediumEmbryoCell SamplingGenetic Analysis

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