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JoVE Journal
Developmental Biology

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Human Blastocyst Biopsy and Vitrification
 

Human Blastocyst Biopsy and Vitrification

Article DOI: 10.3791/59625
July 26th, 2019

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Summary July 26th, 2019

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

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