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Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

DOI:
10.3791/54871-v

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09:35 min

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March 02, 2017

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, , , , , ,
1Ovation Fertility

Chapters

  • 00:05Title
  • 01:10Straw Preparation, Cryodilution and Blastocyst Loading
  • 04:22Cryo-solution Elution/Dilution
  • 06:47Results: Comparative Pregnancy Outcomes
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

MicroSecure Vitrification was developed as a non-commercial, aseptic closed vitrification device system that is compliant with FDA good manufacturing and tissue-handling practices. Due to the withdrawal of hydrophobic plugged embryo straws from the industry, the vitrification procedure was modified to include an inner seal before the standard internal cotton plug.

Tags

Modified Microsecure VitrificationCryopreservationHuman BlastocystsAseptic Closed VitrificationQuality ControlTechnical VariationsFDA-approved ItemsIonomeric Storage StrawFlexipetCryo-dilutionBlastocyst LoadingV1 SolutionV2 SolutionV3 Solution

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