Buccal Swab Sampling
Buccal Swab Sampling - Related Videos
10:03
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
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Cited by 40 •
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A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.
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Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model
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In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.
07:13
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
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A citizen science project was designed to recruit San Diego residents to collect environmental samples for SARS-CoV-2. A multilingual web-based platform was created for data submission using a user-friendly mobile device interface. A laboratory information management system facilitated the collection of thousands of geographically diverse samples with real-time outcome tracking.
02:34
Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture
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2026
Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.
04:45
Swab-based Conjunctival Commensal Bacteria Isolation: A Technique to Isolate Commensal Bacteria from Conjunctiva of Murine Model
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This video describes a swab-based technique to isolate viable commensal bacteria from the ocular conjunctiva. The technique can be used to profile the commensal bacterial microbiome of the conjunctiva, which plays a defensive role against pathogens.
FAQs
The useful material comes primarily from epithelial cells released from the buccal mucosa when the swab is rubbed against the cheek. These cells carry biological material that can support DNA extraction and analysis, while their cellular features can also be examined directly. This cellular origin explains why the sample can serve genetic and cellular investigations without collecting blood.
It offers a noninvasive, painless, inexpensive, and easy-to-perform route to genomic material. The overview indicates that only small amounts of genomic material may be required, making cheek-derived samples practical when a study does not need blood collection. Its convenience can also support studies involving many participants or situations where simpler sampling is preferred.
Suitability depends on the type of information the investigation requires. Buccal cells can provide material for DNA extraction when researchers need genetic information, and they can support examination of cellular characteristics in other studies. The method is especially relevant when the project requires only small amounts of genomic material rather than a more extensive biological sample.
The basic workflow begins with a sterile swab, followed by gentle rubbing against the inside of the cheek to dislodge epithelial cells. The collected cells are then preserved and processed. Depending on the study, processing may lead to DNA extraction for genetic analysis or examination of cellular characteristics, linking collection directly to the intended biological outcome.
Preservation keeps the collected biological material available for subsequent laboratory work, while processing prepares it for the analysis selected by the researcher. The preserved sample may be used to extract DNA or to examine cellular characteristics. These stages therefore connect the simple collection step with reliable access to the genetic or cellular information sought in the study.
The method supports genotyping, human identification, population studies, and investigations of inherited traits or disease-associated variants. These applications use the collected cells as a source of biological material for genetic or cellular analysis. Its practical advantages make it useful when researchers need a straightforward way to obtain samples from participants for such biology-focused investigations.
Because cheek cells can supply material for DNA extraction, the method can support studies that examine inherited traits and disease-associated variants. It also fits population studies and human identification, where researchers may need comparable biological samples from participants. The noninvasive collection approach can make participation more practical while still providing material for genetic analysis.