Calyx Of Held
Calyx Of Held - Related Videos
14:58
Methods for Patch Clamp Capacitance Recordings from the Calyx
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Cited by 6 •
2007
We demonstrate the basic techniques for presynaptic patch clamp recording at the calyx of Held, a mammalian central nervous system nerve terminal.
09:43
Hand-held Clinical Photoacoustic Imaging System for Real-time Non-invasive Small Animal Imaging
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Cited by 10 •
2017
A clinical handheld photoacoustic imaging system will be demonstrated for real-time non-invasive small animal imaging.
09:56
Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time
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Cited by 20 •
2014
We provide herein a detailed description of the experimental protocol for imaging with a newly developed hand-held optoacoustic (photoacoustic) system for three-dimensional functional and molecular imaging in real time. The demonstrated powerful performance and versatility may define new application areas of the optoacoustic technology in preclinical research and clinical practice.
11:52
An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy
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Cited by 5 •
2013
Analysis of vestibular hair cell function is complicated by their location deep within the hardest part of the skull, the petrous temporal bone. Most functional hair cell studies have used acutely isolated hair cells. Here we describe a semi-intact preparation of mouse vestibular epithelium for electrophysiological and two-photon microscopy studies.
03:54
Bioluminescence Imaging for Studying Calcium Transients in Drosophila Brain Structures
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2025
Source: Lark, A. R., et. al., In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin. J. Vis. Exp. (2016)This video demonstrates real-time imaging of calcium dynamics using Green fluorescent protein-aequorin bioluminescence in Drosophila's deep brain structures.
FAQs
Active zones provide repeated sites where an arriving action potential triggers glutamate release from the cochlear nucleus axon terminal. Their large number supports reliable excitation of the medial nucleus of the trapezoid body principal neuron. This organization makes the synapse useful for examining how presynaptic release arrangements contribute to rapid, temporally precise signaling in auditory pathways.
Glutamate provides the excitatory signal that transfers activity from a spherical bushy cell to a principal neuron in the medial nucleus of the trapezoid body. The resulting excitation can participate in rapid feedforward inhibition within auditory circuits. This sequence helps preserve timing information that the brain uses when comparing sound arrival between the two ears.
Its fast and reliable transmission helps auditory circuits retain differences in the timing of activity generated by sounds at the two ears. Excitation through the calyx of Held is linked to feedforward inhibition, which shapes downstream circuit responses. Together, these features support neural computations based on interaural sound timing rather than relying only on signal strength.
The calyx of Held combines a large, accessible synaptic structure with numerous active zones and rapid glutamatergic transmission. Researchers can therefore examine how synaptic signaling changes during closely timed activity while directly recording neuronal responses. These experiments use the synapse as a model for linking release mechanisms to short-term plasticity and precise auditory communication.
Direct electrophysiological recording takes advantage of the calyx of Held's unusually large and accessible structure. Researchers can monitor electrical responses associated with action-potential-driven glutamate release and excitation of the principal neuron. This approach allows measurements of synaptic transmission and timing, making the preparation useful for connecting cellular events with auditory circuit performance.
Studies of the calyx of Held address how neurons achieve reliable communication, how synapses preserve precise timing, and how short-term plasticity alters signal transfer. Its connection to feedforward inhibition also provides a way to examine neural computation in auditory circuits. Consequently, findings from this preparation relate cellular synaptic mechanisms to sound-processing functions in the brainstem.