Cancer Genomics
Cancer Genomics - Related Videos
02:02
Genomics
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2020
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
11:50
Genome-Wide Mapping of Histone Modifications and Transcription Factor Binding Sites in Neuroendocrine Small Cell Lung Cancer Cell Lines Using CUT&RUN
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2026
An optimized Cleavage Under Targets and Release Using Nuclease followed by next generation sequencing (CUT&RUN-seq) protocol is described for neuroendocrine small cell lung cancer cell lines. It enables genome-wide mapping of various histone modifications and transcription factor (e.g. E2F7) binding sites to investigate epigenetic and transcriptional deregulation in SCLC pathobiology.
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
07:50
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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2020
Herein, we describe a procedure for genome-wide analysis of DNA methylation in gastrointestinal cancers. The procedure is of relevance to studies that investigate relationships between methylation patterns of genes and factors contributing to carcinogenesis in gastrointestinal cancers.
FAQs
These alterations can disrupt genes, change the number of genomic copies, or rearrange DNA segments, potentially affecting biological pathways that regulate cancer behavior. Cancer genomics examines their combined patterns rather than treating every change as equivalent. This distinction helps researchers identify alterations associated with tumor development or progression and separate potentially important findings from broader genomic variation.
Comparing tumor DNA or RNA with normal tissue helps identify changes specifically associated with the cancer rather than features present in the patient’s unaffected cells. This comparison provides a reference for interpreting mutations, copy-number alterations, structural variants, and gene-activity changes. The resulting tumor-specific profile can support classification and the search for clinically relevant biomarkers.
Individual genomic changes can influence related genes and processes within biological pathways, creating patterns that are more informative than isolated alterations. By examining these pathway-level effects, researchers can connect genomic findings with cancer development or progression. This analysis also helps reveal molecular vulnerabilities that may guide drug discovery and the design of treatments aimed at specific tumor features.
A typical analysis begins with tumor DNA or RNA and, when available, a normal-tissue comparison. Sequencing generates genomic data, while bioinformatic analysis organizes and interprets mutations, copy-number alterations, structural variants, and changes in gene activity. Researchers then evaluate these results to classify the tumor, identify altered pathways, and assess biomarkers linked with prognosis or treatment response.
Tumor profiling is useful when researchers need a molecular description of a cancer rather than relying only on its broader biological classification. Cancer genomics can identify the genomic alterations and gene-activity patterns present in tumor material, helping distinguish tumor types or subtypes. These profiles support studies of cancer biology, therapeutic response, and molecularly informed treatment strategies.
Cancer genomics connects tumor-specific alterations with potential therapeutic opportunities. Biomarkers associated with treatment response can help researchers investigate which molecular features correspond to particular outcomes, while identified pathway changes can expose possible vulnerabilities for drug development. In biology and clinical research, these findings support treatments designed to target specific molecular characteristics rather than treating every tumor as biologically identical.