Cell Surface Markers
Cell Surface Markers - Related Videos
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
09:38
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
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Cited by 38 •
2011
Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Cited by 1 •
2019
Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
FAQs
A combination of markers can distinguish cells that share one surface feature but differ in identity, state, or function. Researchers compare marker patterns rather than relying on one signal, improving the separation of cellular populations during profiling. This approach is especially useful when studying mixed samples, where related cell types may display overlapping individual markers.
The molecular role of a marker influences what its presence may indicate. Receptors can reflect a cell’s ability to respond to specific ligands, adhesion molecules can relate to interactions with neighboring cells, and recognition signals can support identification by antibodies. Interpreting these functions helps connect marker patterns with cellular behavior rather than treating them as labels alone.
Marker expression responds to biological conditions, including development, activation, disease, and environmental cues. Consequently, a cell’s surface profile may provide information about its current state as well as its identity. Tracking these changes allows researchers to examine transitions such as differentiation or activation and to relate altered profiles to disease-associated or experimentally induced conditions.
Researchers first select a set of surface markers relevant to the populations under study, then profile those markers to identify contrasting cellular groups. Flow cytometry supports marker-based analysis, while magnetic-activated cell sorting supports physical isolation of selected cells. Together, these approaches connect phenotypic identification with the recovery of populations for further biological investigation.
Marker profiling is useful when samples contain multiple cell populations or when cells change during a biological process. It supports studies of cellular differentiation, immune-cell population analysis, and the identification of disease-associated states. By revealing differences in surface features, the approach helps researchers follow development and characterize complex biological samples.
Applications span development, cancer, immunology, and regenerative medicine. Marker analysis can help characterize abnormal or changing cell populations, support more precise diagnostic investigations, and guide the selection of cells for cell-based therapies. Its value comes from linking measurable surface profiles with decisions about cellular identity, state, and experimental or therapeutic use.