Host Cell Surface
Host Cell Surface - Related Videos
10:41
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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Cited by 14 •
2012
In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.
04:15
Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System
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2025
This video demonstrates a permeable membrane insert system to investigate the impact of bacterial toxins on host cells in a controlled and isolated manner. This approach allows for the selective exposure of the cells to the toxins while maintaining a physical separation that mimics the natural host-pathogen interaction.
03:41
Isolation of Intracellular Pathogenic Bacteria from Infected Host Cells
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2026
Source: Sigal, N., et al. RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells. J. Vis. Exp. (2016).This video demonstrates the isolation of internalized pathogenic bacteria from infected bone marrow-derived macrophages (BMDMs). Bacteria are allowed to attach to host cells, and unattached bacteria are washed away. After internalization, an antibiotic is added to eliminate non-internalized bacteria. The host cells are lysed, centrifuged, and the supernatant is...
04:06
Assessing Bacteria-Host Cell Interactions Using Biomimetic Beads
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2025
Source: Stones, D. H., et al. Biomimetic Materials to Characterize Bacteria-host Interactions. J. Vis. Exp. (2015)This video demonstrates the use of bacteriomimetic beads to quantify bacterial adhesion to host epithelial cells under competitive conditions. It outlines the steps involved in treating the cells, lysing them, and plating serial dilutions to measure colony-forming units.
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
FAQs
Receptor binding detects extracellular signals or microbial ligands and initiates intracellular signaling. These signals can change gene expression, reorganize the cytoskeleton, or regulate membrane trafficking. The resulting response depends on the recognized ligand and the signaling pathways engaged, allowing a cell to adjust its behavior during communication, environmental interaction, or pathogen exposure.
Surface-associated carbohydrates contribute to molecular recognition and interactions at the cell boundary. Their presence helps determine how cells contact surrounding structures and how microbial ligands are encountered. In host-pathogen studies, these carbohydrates are therefore part of the interface that can influence attachment, signaling, and subsequent changes in cellular behavior.
Lipids form the membrane environment, while embedded proteins provide specialized functions such as signal recognition, adhesion, and regulated exchange. Their combined organization creates a responsive interface rather than a passive boundary. Changes in receptor activity or membrane organization can affect cytoskeletal structure, intracellular signaling, and trafficking of materials across or within the membrane system.
Surface markers are detectable features used to distinguish or identify cell types, whereas receptor profiles describe the set and distribution of receptors present at the surface. Examining both can provide a more informative molecular signature. This information supports cell characterization and helps researchers compare normal, diseased, or experimentally modified populations.
Surface receptors and adhesion molecules show how cells receive external information and attach to neighboring cells or surrounding structures. Their activity can influence gene expression and cytoskeletal organization, linking molecular recognition to cellular behavior. Studying these interactions helps explain how communication and adhesion contribute to the organization of tissues.
Microbial ligands can bind host-surface receptors or other molecular features, triggering signaling and sometimes pathogen entry. Investigating these interactions identifies how recognition at the cell boundary leads to intracellular consequences. The findings help connect molecular attachment with changes in trafficking, cytoskeletal organization, and gene expression during infection-related processes.
Surface markers and receptor profiles support cell identification, disease research, drug development, and targeted delivery strategies. Researchers can use these molecular features to distinguish cell populations, examine altered surface behavior in disease, or identify cellular entry points for therapeutic approaches. The same information also helps evaluate how treatments interact with specific cell types.