Intestinal Stem Cells
Intestinal Stem Cells - Related Videos
01:23
Renewal of Intestinal Stem Cells
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2023
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
07:48
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Cited by 3 •
2015
Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
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Cited by 31 •
2007
01:33
Adult Stem Cells
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2019
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
FAQs
Wnt and R-spondin signals are key components of the surrounding intestinal stem cell niche. They help regulate the balance between continued stem-cell maintenance and production of descendant cells. Because this signaling environment supports epithelial renewal, changes in these pathways can affect how effectively the intestine replaces cells and responds to tissue damage.
The resulting transit-amplifying progenitors expand the available pool of cells before entering differentiated epithelial lineages. Their descendants can become absorptive or secretory cells, allowing the intestinal epithelium to perform different functions while preserving a source of self-renewing cells. This progression connects stem-cell activity with both tissue composition and epithelial function.
Intestinal stem cell activity must remain coordinated with the signals that control renewal and differentiation. If this regulation becomes abnormal, epithelial maintenance and repair may be disturbed, and the altered behavior can contribute to intestinal disease or tumor formation. Studying these changes helps connect stem-cell biology with pathological processes rather than normal turnover alone.
Researchers examine intestinal stem cells both within living tissue and in organoid cultures, which provide experimental systems for studying epithelial organization and renewal. Comparing these settings helps relate cell behavior to the intestinal environment while also supporting controlled investigation of stem-cell maintenance, differentiation, and responses associated with regeneration.
Research can show how intestinal stem cells support epithelial regeneration after injury, not only routine replacement of cells. This makes them relevant to understanding how the gut restores its protective barrier when tissue is damaged. Findings may also inform regenerative medicine by identifying cellular processes that maintain or rebuild intestinal epithelium.
These cells connect several areas of biology because their activity influences development, intestinal physiology, tissue repair, and cancer biology. Their normal role in maintaining a rapidly renewed epithelium provides a basis for interpreting what happens when renewal signals or differentiation become dysregulated. Organoid and in vivo studies help investigate these relationships in complementary ways.