Mammary Tissue Massage

Mammary tissue massage is a controlled manual technique that applies gentle pressure and movement to the breast or mammary gland, making it relevant to mammalian physiology, lactation, and tissue mechanics. Repeated strokes or compression deform the surrounding tissue, influence local fluid movement, and stimulate sensory pathways that can contribute to milk ejection during lactation. In biology, the technique can help examine mammary-gland responses to mechanical stimulation, support milk expression protocols, and provide context for studying tissue structure and function. Its effects depend on pressure, timing, tissue condition, and the biological state of the mammary gland.

Mammary Tissue Massage - Related Videos

Research

JoVE EoE - Breast Cancer

Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads

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2023

This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

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Cited by 21 •

2018

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Intraductal Delivery to the Rabbit Mammary Gland

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Cited by 9 •

2017

Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.

Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes

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Cited by 8 •

2017

This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.

Mammary Epithelial Transplant Procedure

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Cited by 23 •

2010

This article demonstrates the procedure developed by DeOme KB et al. (1959) and the sparing procedure developed by Brill B et al. (2008) for clearing the 4th inguinal mammary fat pad of a pubescent mouse in preparation for transplantation of mammary fragments, mammary epithelial cells, or mammary tumor cells.

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