Microprep Analysis
Microprep Analysis - Related Videos
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
10:39
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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Cited by 3 •
2022
Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
06:24
Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
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Cited by 16 •
2020
A number of different procedures for preparing nanoparticles for surface analysis are presented (drop casting, spin coating, deposition from powders, and cryofixation). We discuss the challenges, opportunities, and possible applications of each method, particularly regarding the changes in the surface properties caused by the different preparation methods.
14:11
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Cited by 21 •
2016
We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
FAQs
The workflow links disruption or lysis with separation, concentration, purification, and measurement so that target molecules or cellular features remain available for analysis despite limited input. Miniaturization also reduces reagent consumption and can decrease handling-related sample loss. Its success depends on matching each preparation step to the target and preserving enough material for a reliable analytical readout.
These steps help distinguish the desired biological target from unwanted sample components and increase its suitability for measurement. Separation can reduce interfering material, concentration can improve detectability, and purification can support a cleaner analytical readout. Their order and effectiveness influence recovery, so the preparation must be evaluated together with the method used to examine DNA, RNA, proteins, or cellular features.
Small-scale workflows can be sensitive to contamination and sample loss because only limited material is available for analysis. Careful handling helps prevent unwanted biological material from entering the preparation, while validation checks whether the analytical method produces dependable results for the prepared sample. Without these controls, an apparent signal may be difficult to distinguish from preparation-related error or interference.
A typical sequence begins by disrupting or lysing the biological sample, followed by separating relevant material from unwanted components. The target may then be concentrated and purified before an analytical readout examines molecules or cellular features. Researchers should define the target and measurement method early, because preparation choices determine how much usable material reaches the final analysis.
This approach is valuable when specimens are precious, sample volume is restricted, or experimental time and reagent use must be minimized. It also supports screening designs that require many parallel measurements. Applications can include DNA, RNA, protein, and cell-based investigations, allowing researchers to examine multiple limited samples without requiring the scale of a conventional preparation.
Results should be considered in light of the available input, preparation efficiency, contamination controls, and the suitability of the analytical readout. A detectable signal does not by itself establish that recovery or purification was adequate. Comparing outcomes with appropriate validation helps determine whether observed differences reflect biological variation or limitations introduced during the small-scale preparation.