Multidrug Resistant Fungi
Multidrug Resistant Fungi - Related Videos
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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
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Cited by 32 •
2008
The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture method.
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Filamentous Fungi
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Fungi are heterotrophic eukaryotic organisms, and with the exception of yeasts, are aerobic. They are abundant in surface soils and are important for their role in nutrient cycling and the decomposition of organic matter and organic contaminants. White rot fungi (phanerochaete chryosporium) for example, (Figure 1) are known to degrade aromatics. Figure 1. White rot on...
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Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
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In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.
01:29
Overview of Fungi
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2025
Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...
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In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging
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Cited by 20 •
2017
Infections caused by multidrug-resistant (MDR) bacterial strains have emerged as a serious threat to public health, necessitating the development of alternative therapeutics. We present a protocol to evaluate the effectiveness of antimicrobial blue light (aBL) therapy for MDR Acinetobacter baumannii infections in mouse burns by using bioluminescence imaging.
FAQs
Resistance can emerge when mutations or other genetic changes modify antifungal drug targets. A treatment may then bind less effectively, allowing fungal cells to survive exposure. Because different drug classes act through different targets, changes affecting more than one target or resistance pathway can contribute to multidrug resistance and complicate selection of effective therapy.
Increased drug efflux lowers intracellular drug activity by moving antifungal compounds away from their cellular sites of action. This mechanism can work alongside altered targets or protective structures, producing a stronger resistance phenotype than any single change alone. Identifying which mechanisms predominate helps researchers interpret susceptibility results and prioritize new or combination treatments.
Strengthened fungal cell structures can reduce the effectiveness of antifungal agents by providing additional protection against drug activity. Biofilms can also help fungal populations persist in a protected state, making control more difficult. Studying these features connects cellular biology with treatment outcomes and can reveal targets for therapies designed to weaken protection rather than only attack the fungus directly.
When antifungal exposure eliminates susceptible fungal cells more effectively than cells carrying protective traits, those surviving traits can become more prominent in the population. This selection process may favor changes involving drug targets, efflux, cell structures, biofilms, or intracellular drug activity. Understanding selection helps explain why resistance can increase during continued treatment pressure.
Antifungal susceptibility testing evaluates how a fungal isolate responds to available antifungal agents. The resulting information helps researchers and clinicians recognize reduced drug activity and compare options when resistance is suspected. In studies of multidrug-resistant fungi, these tests also support monitoring of treatment challenges and evaluation of whether new or combination approaches may be useful.
Genomic surveillance examines genetic changes associated with resistance across fungal isolates or populations. By tracking these changes, researchers can investigate how resistance mechanisms arise and monitor their distribution over time. This biological information complements susceptibility testing and supports broader efforts to recognize emerging resistance, assess transmission concerns, and guide infection-control planning.
Resistance studies connect mechanism-based findings with practical strategies for limiting treatment failure and spread. Infection-control planning can use surveillance and susceptibility information, while drug-development research can target resistance pathways or evaluate combinations that retain activity. These approaches are also relevant to biology because they translate cellular and genetic observations into methods for managing fungal infections.