Nano Lc-ms
Nano Lc-ms - Related Videos
10:27
Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition
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Cited by 15 •
2013
We describe the experimental method to deposit nanostructured oxide thin films by nanosecond Pulsed Laser Deposition (PLD) in the presence of a background gas. By using this method Al-doped ZnO (AZO) films, from compact to hierarchically structured as nano-tree forests, can be deposited.
06:52
Nanocrystalline Alloys and Nano-grain Size Stability
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2023
Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT Alloys with grain size less than 100 nm are known as nanocrystaline alloys. Due to their enhanced physical and mechanical properties, there is an ever-increasing demand to employ them in various industries such as semiconductor, biosensors and aerospace. To improve the processing and application of nanocrystalline alloys, it is necessary to develop close to 100%...
11:10
Fabrication and Operation of a Nano-Optical Conveyor Belt
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2015
The scalability and resolution of conventional optical manipulation techniques are limited by diffraction. We circumvent the diffraction limit and describe a method of optically transporting nanoparticles across a chip using a gold surface patterned with a path of closely spaced C-shaped plasmonic resonators.
10:34
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
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Cited by 3 •
2017
We present a protocol to functionalize glass with nanometric protein patches surrounded by a fluid lipid bilayer. These substrates are compatible with advanced optical microscopy and are expected to serve as platform for cell adhesion and migration studies.
13:29
Biodistribution of Nano-drug Carriers: Applications of SEM
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Nanoparticles have been increasingly used research towards targeted drug delivery and controlled drug release. While most of these particles have been developed as polymeric or liposomal particles because of their biocompatibility, there is a trend in current research toward the use of metallic and magnetic nanoparticles. These metallic nanoparticles...
FAQs
The packed chromatographic column separates peptides, proteins, or metabolites according to their chemical properties before they reach the mass spectrometer. This reduces the complexity of the material entering the detector, allowing individual components to be examined through their mass-to-charge ratios and fragmentation patterns. The separation step therefore complements mass analysis by improving resolution within complex biological samples.
Electrospray ionization transfers separated molecules into the mass spectrometer as ions, enabling measurement of their mass-to-charge ratios. Fragmentation adds structural information by breaking ions into characteristic pieces. Considering both measurements helps distinguish molecular components and supports analysis of peptides, proteins, and metabolites when mass alone may not provide sufficient information.
Very low solvent flows support analysis when only a small amount of biological material is available. By carrying samples through the chromatographic system in low volumes, the technique helps conserve limited material while maintaining high sensitivity. This is particularly valuable for experiments focused on subtle molecular differences, where sample scarcity and detection capability can strongly affect the study.
A typical workflow moves a biological sample through a packed nano-scale chromatographic column, where its molecular components separate by chemical properties. The separated components then enter an electrospray ionization source and proceed to the mass spectrometer. Researchers interpret the resulting mass-to-charge measurements and fragmentation patterns to characterize the molecules present in the sample.
In biology, Nano LC-MS supports several complementary areas, including proteomics, metabolomics, post-translational modification analysis, and biomarker research. These applications allow investigators to examine proteins, peptides, metabolites, or molecular modifications in complex samples. Its low sample consumption is useful when biological material is limited, while its sensitivity supports detection of changes associated with health, disease, or experimental conditions.
The combined separation and detection approach can reveal differences in molecular composition and help characterize complex biological systems. Measurements may support comparisons across health states, disease conditions, or experimental treatments by identifying changes in peptides, proteins, metabolites, or post-translational modifications. The resulting molecular profiles can also contribute to biomarker research and the interpretation of subtle biological responses.