Nasal Nebulization
Nasal Nebulization - Related Videos
02:46
A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
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Cited by 4 •
2023
Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin nebulization.
07:28
Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System
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Cited by 11 •
2017
The inhalation unit described herein can generate, sample for characterization, and uniformly deposit a drug aerosol in the lungs of rodents. This enables the pre-clinical determination of the efficacy and safety of drug doses deposited in the lungs; key data enabling clinical inhaled drug development.
15:04
Modeling and Simulations of Olfactory Drug Delivery with Passive and Active Controls of Nasally Inhaled Pharmaceutical Aerosols
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Cited by 18 •
2016
This manuscript reviews the modeling and simulations of different protocols to deliver medications to the olfactory region in image-based nasal airway models. Multiple software modules are used to develop the anatomically accurate nose model, generate computational mesh, simulate nasal airflows, and predict particle deposition at the olfactory region.
05:50
Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and Functional Testing
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2026
This protocol presents Rayleigh breakup as a mild process for aerosolizing non-viral mRNA vectors. Regional deposition for aerosolized mRNA vectors is evaluated by the Alberta Idealized Nasal Inlet (AINI) model. Green fluorescence protein (GFP) expression in human lung cells represents maintenance of physicochemical and biological functions of post-aerosolized mRNA vectors.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 134 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
FAQs
Droplet size helps determine where aerosolized material deposits within the nasal passages and how it interacts with the mucosal surface. Along with formulation properties and breathing pattern, it affects distribution and absorption. Researchers therefore consider these variables when examining whether a molecule remains localized in nasal tissue or contributes to systemic exposure.
Compressed air, vibration, and other atomization processes generate the aerosol from a liquid formulation, but the resulting droplets may differ in characteristics relevant to deposition. This makes the nebulizer mechanism an important experimental variable. Selecting or comparing atomization approaches can help researchers evaluate how delivery conditions influence contact with nasal mucosa.
The nasal route provides a way to investigate how therapeutic molecules interact with nasal tissue and may enter the body without injection. In biology and biomedical research, this supports nose-to-brain studies alongside investigations of mucosal barriers. The approach helps examine delivery behavior at the interface between nasal exposure and broader biological distribution.
A typical experiment begins with a liquid formulation, places it in a nebulizer, and uses an atomization process such as compressed air or vibration to produce droplets. The aerosol is administered through the nose while the breathing pattern is considered. Researchers then assess deposition, mucosal interaction, or absorption according to the study objective.
The key experimental inputs are the liquid formulation, the nebulizer’s atomization process, droplet size, and the subject’s breathing pattern. These factors collectively influence how the aerosol travels through the nasal passages and where it deposits. Controlling or documenting them is important when comparing nasal tissue exposure, absorption, or systemic treatment outcomes.
Researchers can use this technique to study respiratory exposure, mucosal barriers, and the movement of therapeutic molecules after nasal administration. It also provides a practical route for evaluating localized or systemic treatment without injection. Results can reveal how formulation and delivery conditions shape nasal deposition and the interaction between molecules and nasal tissue.