Plant Cell Cytosol

Plant cell cytosol is the aqueous, soluble phase of the cytoplasm that surrounds organelles and supports essential cellular activities. It contains water, ions, metabolites, proteins, and enzymes, allowing molecules to diffuse, interact, and participate in processes such as glycolysis, protein synthesis, and intracellular signaling. In plant cells, the cytosol also coordinates transport between organelles, responds to environmental cues, and helps regulate cellular pH and osmotic balance. Understanding its composition and dynamics is important for studying plant growth, metabolism, stress responses, and the molecular basis of adaptations to changing conditions.

Plant Cell Cytosol - Related Videos

Research

JoVE Journal - Biology

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

0 Views •

Cited by 40 •

2013

Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

0 Views •

Cited by 26 •

2012

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

0 Views •

Cited by 20 •

2014

Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.

Assaying Proteasomal Degradation in a Cell-free System in Plants

0 Views •

Cited by 26 •

2014

Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics

0 Views •

Cited by 24 •

2011

We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.

View All Results

FAQs

Related Topics