Pluripotent Cells
Pluripotent Cells - Related Videos
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
11:56
Generation of Mice Derived from Induced Pluripotent Stem Cells
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Cited by 16 •
2012
Generating induced pluripotent stem cell (iPSC) lines produces lines of differing developmental potential even when they pass standard tests for pluripotency. Here we describe a protocol to produce mice derived entirely from iPSCs, which defines the iPSC lines as possessing full pluripotency1.
06:40
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
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Cited by 56 •
2014
We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s disease.
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Cited by 24 •
2014
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
10:49
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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Cited by 38 •
2013
This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.
FAQs
Maintenance depends on coordinated gene-regulatory networks and signaling conditions that support the undifferentiated state. These controls preserve the cells’ developmental potential rather than directing them toward a specialized identity. In experimental systems, changing those regulatory inputs can therefore shift the balance from continued self-renewal toward lineage-specific differentiation.
Changes in signaling cues alter the regulatory state of the cells and guide them toward specialized fates associated with the three embryonic germ layers. The outcome depends on how those conditions influence lineage decisions. This principle allows researchers to study developmental pathways and produce cell populations suited to particular biological questions.
Genomic stability must be addressed because abnormalities can affect how pluripotent cells behave during self-renewal or differentiation and can complicate interpretation of experimental results. It is also relevant to potential therapeutic use, where researchers must distinguish reliably differentiated cells from populations that may behave unpredictably or show unwanted growth.
By maintaining these cells and then guiding them toward lineage-specific identities, researchers can model aspects of how specialized cell types arise from early developmental potential. Embryonic stem cells and induced pluripotent stem cells provide experimental systems for examining human development, including how regulatory networks and signaling conditions influence developmental outcomes.
These two pluripotent cell sources support models of human development, disease mechanisms, and drug responses. Researchers can examine how cells acquire specialized identities, investigate changes associated with disease-related processes, and study how candidate treatments affect relevant cell types. Their shared experimental value comes from combining developmental potential with controlled laboratory differentiation.
Controlled differentiation can generate specialized cell types that may be relevant to regenerative medicine, linking developmental biology with efforts to replace or restore damaged tissues. However, practical progress depends on improving differentiation efficiency, maintaining genomic stability, and reducing the risk of unwanted cell growth. These limitations remain central considerations when evaluating therapeutic applications.