Sc1 Small Molecule
Sc1 Small Molecule - Related Videos
05:58
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
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Cited by 7 •
2009
SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.
02:38
Molecules and Compounds
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2020
Atoms and Molecules Everything in the universe is made up of matter, and matter is composed of a combination of elements. An atom is the smallest unit of an element that retains all properties of the element. For example, a silver coin is made up of silver atoms, and each silver atom retains the unique properties of the element — silver. Elements are pure substances consisting of identical atoms that cannot be broken down into simpler substances by chemical changes. Atomic elements contain...
12:13
Single-Molecule Imaging of Nuclear Transport
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2010
Single molecule microscopy approch provided novel insights into nuclear transport.
12:05
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
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Cited by 8 •
2017
This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.
06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
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Cited by 13 •
2014
Optical tweezers have been used to study RNA folding by stretching individual molecules from their 5’ and 3’ ends. Here common procedures are described to synthesize RNA molecules for tweezing, calibration of the instrument, and methods to manipulate single molecules.
FAQs
SC1 combines inhibition of RasGAP with inhibition of ERK1, targeting two points that influence signaling associated with stem-cell state. RasGAP regulates Ras activity, while ERK1 functions within the MAPK pathway. Acting through both routes provides complementary control rather than relying on a single signaling intervention, helping mouse embryonic stem cells remain undifferentiated.
ERK1 is a kinase in the MAPK pathway, so inhibiting it allows researchers to examine how MAPK-associated signaling affects the balance between self-renewal and differentiation. In the SC1 system, ERK1 inhibition contributes to preservation of an undifferentiated state. This makes the compound useful for connecting pathway activity with changes in stem-cell identity.
RasGAP modulates Ras signaling, providing a regulatory target distinct from ERK1. Inhibiting RasGAP therefore complements ERK1 inhibition and helps explain why SC1 acts through more than one signaling route. Studying this combination allows investigators to assess how coordinated control of signaling can support self-renewal instead of examining MAPK-related regulation in isolation.
SC1 can support mouse embryonic stem-cell self-renewal under serum-free conditions without leukemia inhibitory factor, whereas the overview describes growth-factor-based maintenance as a separate strategy. This distinction lets researchers investigate chemical regulation of stem-cell state and develop defined culture systems in which signaling inhibitors supplement or replace growth-factor-dependent maintenance.
Its reported activity under serum-free culture conditions without leukemia inhibitory factor makes SC1 valuable when researchers want to reduce reliance on those maintenance inputs. Such conditions help isolate the contribution of defined chemical signaling control to self-renewal. The resulting system can support investigations of how culture design influences preservation of an undifferentiated mouse embryonic stem-cell population.
SC1-treated cultures can be used to study pluripotency, signaling control, and the transition between self-renewal and differentiation. Researchers can also examine whether chemical pathway regulation can replace or supplement growth-factor-based maintenance. These applications connect molecular signaling mechanisms with practical culture-system development and with broader questions about how embryonic stem-cell identity is preserved.