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A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

DOI:
10.3791/55923-v

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12:05 min

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October 01, 2017

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,
1Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, 2Dept. of Biological Engineering,Massachusetts Institute of Technology

Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:31Preparation of the biotin-λ-DNA
  • 02:52Coverslip functionalization
  • 04:10Flow cell construction
  • 05:44TIRF Imaging
  • 08:15Data Analysis
  • 11:41Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.

Tags

Keywords: Single MoleculeFlow Stretching AssayDNA TransportBiotin-labeled Lambda-DNAPEG Functionalized CoverslipsHigh Throughput Flow CellTIRF ImagingSingle Particle Tracking

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