Tissue Processing Cassette
Tissue Processing Cassette - Related Videos
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
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Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
09:37
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Cited by 13 •
2015
This protocol describes the detection of class 1 integrons and their associated gene cassettes in foodstuffs.
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
07:48
Working with Human Tissues for Translational Cancer Research
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Cited by 4 •
2015
Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.
04:21
Organotypic 3D Skin Culture: A Cassette-based In Vitro Culture Technique to Regenerate Human Epidermis on Acellular Dermis
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2023
This video protocol describes a cassette-based in vitro culture technique to generate human organotypic skin using epidermal keratinocytes and devitalized human dermis. They mimic the function and physiology of their in vivo tissue counterparts.
FAQs
The perforations permit fixatives, dehydrating agents, clearing agents, and molten paraffin to pass around the specimen while the tissue stays enclosed. This exchange exposes the sample to the solutions needed during preparation without requiring transfer between open containers. The result is a continuous, contained workflow that supports preservation and reduces the chance of losing the specimen.
Maintaining orientation depends on how the specimen is positioned and retained while it moves through fixation, processing, embedding, and sectioning. The cassette keeps the tissue associated with its identifying surface and limits handling during these transitions. That continuity helps technicians produce sections in a consistent relationship to the original specimen, supporting interpretation of tissue structure.
A writable area or barcode connects the physical cassette with sample records throughout the workflow. This linkage helps technicians distinguish specimens as they pass through preparation steps and reduces mix-ups between tissue pieces. In pathology and experimental biology, reliable identification strengthens the connection between the microscopic section, the original sample, and the associated study information.
After tissue is placed in the cassette, it can remain contained as the workflow proceeds through fixation, dehydration, clearing, and paraffin embedding before sectioning. Solutions move through the openings at each relevant stage. Keeping the sample in the same labeled container supports consistent handling and reduces specimen loss during these sequential preparation steps.
They are useful whenever biological tissue must be prepared for microscopic examination while its identity is maintained. Histology and pathology workflows use them to support tissue-structure and disease studies, while experimental biology can apply the same traceable approach to prepared specimens. Their standardized format is especially valuable when consistent processing and reproducibility matter across samples.
By supporting fixation, processing, embedding, and sectioning, the cassette contributes to specimens suitable for microscopic examination. The resulting sections can be used to study tissue structure and disease, provided the tissue remains correctly identified and oriented. In experimental biology, this supports reproducible comparisons among prepared samples rather than treating each specimen as an isolated, undocumented item.