Tracheal Incision

Tracheal incision is a surgical opening made through the wall of the trachea to create access to the airway, most commonly as part of a tracheostomy. The procedure involves exposing the anterior trachea, carefully separating surrounding tissues, and opening selected tracheal rings so that a tube can maintain a direct passage for airflow while bypassing the nose and mouth. In biology and medicine, this technique supports airway management when upper-airway obstruction, injury, or prolonged mechanical ventilation limits normal breathing. Studying tracheal incision also illustrates airway anatomy, tissue repair, inflammation, and the physiological consequences of altering respiratory pathways.

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Research

JoVE EoE - Large Animal Models

Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea

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2025

This video demonstrates the surgical creation of an opening in the rabbit trachea to insert a tracheal cannula for artificially ventilating the lungs.

Direct Tracheal Instillation of Solutes into Mouse Lung

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Cited by 54 •

2010

Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice.

In vitro Measurements of Tracheal Constriction Using Mice

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Cited by 15 •

2012

Transgenic mice have been extremely useful in ascribing physiological function to genes. As such, research in general, and functional studies of airway, in particular, have undergone a remarkable shift toward murine models. Here we provide protocols for in vitro trachea constriction studies to evaluate smooth muscle function in murine airway.

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

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Cited by 11 •

2013

Here, we present a method for light microscopy analysis of tracheal terminal cells in Drosophila larvae. This method allows for quick examination of branch and lumen morphology in whole animals and would be useful for analysis of individual mutants or screens for mutations affecting terminal cell development.

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

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Cited by 7 •

2010

This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.

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