In Vitro Condition
In Vitro Condition - Related Videos
15:08
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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Cited by 13 •
2017
Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.
11:22
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
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Cited by 29 •
2013
Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.
10:24
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
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Cited by 13 •
2015
To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
07:18
Bone Conditioned Medium: Preparation and Bioassay
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Cited by 13 •
2015
We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.
FAQs
Its main value is variable control: investigators can adjust nutrient composition, temperature, pH, oxygen availability, extracellular matrix, and signaling compounds or drugs while observing defined cellular responses. Holding most factors constant and changing one or more selected inputs helps connect conditions with proliferation, migration, invasion, gene expression, or treatment sensitivity.
Because it is a regulated feature rather than an incidental background variable, extracellular matrix can be included deliberately in experimental design. This lets investigators examine cancer-cell behavior under different modeled surroundings and determine whether observed changes in migration, invasion, proliferation, or treatment sensitivity accompany that condition. This is especially relevant when moving toward three-dimensional systems designed to represent tumor behavior more closely.
These variables help define the conditions under which cancer cells, tissues, or biological molecules are examined. Researchers can regulate them separately or in combination, then assess outcomes such as proliferation, gene expression, or treatment sensitivity. This approach supports controlled comparisons and helps distinguish responses associated with the experimental environment from responses associated with an added signaling compound or drug.
They reduce the complexity of a whole-animal system, allowing investigators to focus on selected variables and cellular responses. That narrower setting improves experimental precision and supports direct measurement of proliferation, migration, invasion, gene expression, and treatment sensitivity. The controlled approach also motivates development of more representative three-dimensional and patient-derived systems for cancer studies.
An experiment begins by selecting the biological material, such as cells, tissues, or biological molecules, and defining the variables to regulate. Researchers then set nutrient composition, temperature, pH, oxygen availability, and extracellular matrix, with signaling compounds or drugs added when relevant. They measure the chosen cancer-related responses under these controlled settings.
Drug or signaling-compound exposure can be evaluated through changes in proliferation, migration, invasion, gene expression, and treatment sensitivity. These readouts allow investigators to compare cellular responses across controlled conditions and support therapeutic screening. The same measurements can also contribute to biomarker evaluation by linking an experimental response with a candidate cancer-related indicator.
Three-dimensional cultures are useful when researchers need a model intended to represent tumor behavior more closely than simpler in vitro arrangements. They extend controlled experimentation while preserving the ability to examine cellular responses to signaling compounds or drugs. In cancer research, these systems support studies of behavior and treatment sensitivity within increasingly representative experimental models.
Patient-derived tumor models help extend in vitro studies toward tumor material obtained from individual patients. Within controlled conditions, researchers can examine cancer-related responses and treatment sensitivity in these models while retaining the experimental precision of laboratory systems. Their use supports the development of more representative approaches for cancer biology, therapeutic screening, and biomarker evaluation.