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Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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JoVE Journal Neuroscience
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

DOI:
10.3791/55108-v

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15:08 min

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October 21, 2017

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, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Chapters

  • 00:00Title
  • 01:01Isolation of Primary Astrocytes from Rat Cortices
  • 09:25Oxygen-Glucose Deprivation (OGD) of Primary Astrocytes
  • 13:40Representative Results
  • 14:08Conclusion

Summary

Automatic Translation

Automatic Translation

Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.

Tags

AstrocyteAstrocyte ReactivityAstrocyte ProliferationIn VitroIschemic-like ConditionsOxygen Glucose Deprivation (OGD)GFAPPrimary Astrocytic Cell CulturePup Rat CorticesCerebral HemispheresCorticesMeningesDissection MicroscopeComplete DMEM Media

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