Silicone Ring Seeding
Silicone Ring Seeding - Related Videos
02:33
Seed Structure and Early Development of the Sporophyte
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2020
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo. The embryo contains a double set of chromosomes, one set from each parent. Fertilization of the haploid egg by the haploid sperm gives rise to the zygote, which develops into the embryo. The endosperm is a...
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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.
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Silicon Microchips for Manipulating Cell-cell Interaction
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This article describes an experimental approach for dynamic regulation of cell-cell interactions between adherent cells on a micrometer scale. Manipulation of intercellular communication between hepatocytes and stromal cell is demonstrated. The developed platform enables investigation of cell-cell interactions in a variety of biological processes, including development and pathogenesis.
08:52
A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
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Cited by 21 •
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We present the procedure to assemble a seed coat bedding assay (SCBA) from Arabidopsis thaliana seeds. The SCBA was shown to be a powerful tool to explore genetically and in vitro how the endosperm controls seed germination in dormant seeds and in response to light cues. The SCBA is in principle applicable under any situation where the endosperm is suspected to influence embryonic growth.
03:42
Introduction to Seed Plants
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2026
Most plants are seed plants—characterized by seeds, pollen, and reduced gametophytes. Seed plants include gymnosperms and angiosperms. Gymnosperms—cycads, ginkgo biloba, gnetophytes, and conifers—typically form cones. The pollen cones contain male gametophytes. The ovulate cones contain female gametophytes and form exposed seeds when fertilized. Angiosperms, the most diverse and ubiquitous group of land plants, form flowers, and fruit. Like the cones of gymnosperms, the flowers and fruit of...
FAQs
While the ring is in place, cells are confined to a defined area on the culture surface. After attachment, removing the barrier leaves a cell-free region with a consistent geometry. Researchers can then compare how quickly and extensively cells move or spread into that region over time, using the same initial spatial arrangement across conditions.
The removable ring creates the gap through a defined physical boundary rather than an approximate line drawn by hand. This standardized geometry can reduce variation in the starting region, making comparisons between cancer-cell samples, treatments, or microenvironmental conditions more reproducible. The main value is consistency of assay setup, not simply the presence of an empty area.
Attachment provides the point at which the barrier can be removed without losing the intended seeded arrangement. If the cells have not attached, the post-removal cell-free region may not represent a comparable starting condition. Once attachment is established, subsequent changes in the region can be monitored as migration, spreading, or wound closure over time.
Follow-up imaging or observation can focus on movement into the cell-free region, expansion of the seeded population, or closure of the initial gap. These readouts correspond to migration, spreading, and wound closure, respectively, and allow tumor-cell behavior to be compared across experimental conditions. The technique therefore links a controlled starting geometry to time-dependent behavioral measurements.
Researchers first place the silicone ring on the culture surface, add cells within its boundary, allow attachment, and then remove the ring. They monitor the remaining cell-free region over time. Maintaining the same sequence across samples helps ensure that observed differences reflect cell behavior or experimental conditions rather than inconsistent initial geometry.
The key setup elements are a culture surface, a removable silicone ring, and a cell suspension placed inside the ring. The ring’s boundary determines the seeded area, while the remaining surface becomes the reference region after removal. Keeping this geometry consistent supports side-by-side assessment of migration, spreading, or closure.
It is useful when investigators need to compare tumor-cell motility, invasion, or responses to drugs and microenvironmental cues from a common spatial starting point. By following the same cell-free region over time, they can assess how experimental conditions alter behavior. Its controlled geometry also supports comparative studies designed to improve reproducibility.