Gene Transfer Electroporation
Gene Transfer Electroporation - Related Videos
06:10
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
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Cited by 2 •
2012
A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.
22:02
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
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Cited by 23 •
2012
The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.
09:03
Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
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Cited by 17 •
2015
We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.
02:30
Gene Transfer into Schwann Cells of the Rodent Sciatic Nerve via Electroporation
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2025
Source: Ino, D. et. al., In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation. J. Vis. Exp. (2016)This video demonstrates in vivo gene transfer to Schwann cells of the rodent sciatic nerve using electroporation. A plasmid DNA–dye solution is injected into the nerve, and electric pulses facilitate DNA entry. The DNA later integrates into the cell’s genome, enabling successful gene transfer in Schwann cells.
05:40
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Cited by 4 •
2016
Here, we present an in vivo technique for gene transfer to Schwann cells (SCs) in the rodent sciatic nerve. This simple technique is useful for investigating signaling mechanisms involved in the development and maintenance of myelinating SCs.
FAQs
The temporary pores created by the electric field provide a short-lived entry route for DNA or other nucleic acids. Their transient nature allows the membrane to reseal after material enters the cell, while the delivered genetic material can subsequently alter gene activity. This sequence connects the electrical treatment to measurable changes in cellular function or developmental behavior.
Transient delivery allows investigators to change gene activity in living cells, embryos, or developing tissues without treating the genetic manipulation as an isolated molecular event. Researchers can then examine how that change affects signaling pathways, cell fate, or tissue formation. The approach therefore helps connect gene function with the visible or functional outcomes of development.
Gene Transfer Electroporation can introduce DNA or other nucleic acids, giving researchers flexibility in how they modify cellular activity. The delivered material may be used to influence gene expression and then assess resulting changes in cell behavior or development. This range makes the technique adaptable to experiments focused on gene function, signaling, or tissue formation.
A typical workflow places the selected genetic material in living cells, embryos, or developing tissue and applies brief electrical pulses. The electric treatment creates temporary membrane access, after which the membrane reseals and the transferred material may be expressed. Researchers then evaluate altered gene activity, cellular function, signaling, cell fate, or tissue formation.
The method can be applied across several developmental contexts, including embryos, cultured cells, and developing tissues. This breadth allows experiments to examine gene function at different levels, from cellular responses to tissue-level organization. Its use in multiple systems also supports comparisons between molecular changes and developmental outcomes in distinct biological settings.
Results may reveal whether changing the activity of a selected gene affects cellular function, signaling pathways, cell fate, or tissue formation. By relating genetic manipulation to these developmental outcomes, researchers can investigate links between molecular mechanisms and the progression of development. The technique is especially useful when experiments require targeted manipulation and flexible genetic delivery.