Mouse Ovarian Tissue
Mouse Ovarian Tissue - Related Videos
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
04:30
Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
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Cited by 15 •
2018
Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.
03:40
Orthotopic Inoculation of Ovarian Cancer Cells into Murine Ovary: A Procedure to Establish an Ovarian Cancer Mouse Model to Study Peritoneal Dissemination of Cells
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2023
This video describes the protocol for creating a murine experimental model to study tumor development and peritoneal metastasis. This ovarian cancer model is generated by the orthotopic inoculation of human epithelial ovarian cancer cells into the ovaries of a female mouse.
FAQs
Folliculogenesis provides a developmental framework for examining how oocytes progress within follicles while surrounding somatic cells support their changing needs. Tracking this coordination helps researchers connect follicle formation with ovarian maturation rather than studying germ cells in isolation. It also provides a way to investigate how disruptions in developmental signals may influence fertility or reproductive aging.
Granulosa and theca cells contribute structural support, nutrients, and hormone-responsive signaling around developing oocytes. Their activities create a local environment in which follicle development can proceed and oocyte maturation can be studied in context. Examining these cell interactions helps clarify how somatic support influences germ-cell development and how the follicle functions as a coordinated developmental unit.
Oocyte development depends on interactions with the surrounding follicle rather than on germ-cell activity alone. The local microenvironment includes support and signaling from associated somatic cells, which can influence follicle formation and maturation. Studying these relationships in mouse ovarian tissue allows developmental biologists to assess how cellular context shapes reproductive development and may affect later fertility-related outcomes.
The tissue provides a connected setting for examining several developmental stages, including germ-cell specification, follicle formation, and ovarian maturation. Researchers can use this progression to relate early developmental signals to later organization and function. Linking these stages is valuable because it shows how initial germ-cell decisions and subsequent interactions with somatic cells contribute to the developing ovary.
Tissue culture can help researchers examine ovarian development while retaining the relationships among oocytes, follicles, and supporting somatic cells. In this setting, developmental signals can be investigated in relation to follicle formation and maturation. The approach therefore supports analysis of how the ovarian microenvironment influences reproductive development and can provide insight into mechanisms associated with fertility or ovarian disorders.
Genetic analysis can be combined with mouse ovarian tissue studies to investigate how particular developmental signals influence germ-cell specification, follicle formation, or ovarian maturation. Tissue context helps connect genetic effects with interactions among oocytes and somatic cells. Together, these approaches can reveal links between developmental regulation, fertility, reproductive aging, and ovarian disorders without isolating genetic changes from tissue organization.
Researchers use this model when they need to relate ovarian development to outcomes such as fertility or reproductive aging. Because the tissue supports analysis of folliculogenesis, cellular interactions, and developmental signals, it can help identify how changes in these processes affect reproductive function. The same framework also supports investigation of ovarian disorders that arise from altered development or tissue regulation.
Its value extends to developmental biology because the ovary offers a model for studying coordinated organ formation, cellular communication, and interactions between specialized cells and their microenvironment. Findings from tissue culture and genetic analysis can therefore contribute to broader understanding of mammalian organ development. The model connects reproductive tissue organization with general principles of developmental signaling and maturation.