Electrowetting Effect

The electrowetting effect is the electrically controlled alteration of a liquid’s apparent wettability on a solid surface, typically observed as a change in contact angle. Applying a voltage across a conductive droplet, insulating dielectric layer, and electrode modifies the interfacial energy at the solid-liquid boundary, causing the droplet to spread or retract; the dielectric layer prevents direct current flow. This precise control of droplet motion and shape supports engineering applications including digital microfluidics, lab-on-a-chip systems, variable-focus lenses, and electronic displays. Electrowetting enables compact, low-power fluid handling and optical control without mechanical pumps or moving parts.

Electrowetting Effect - Related Videos

Research

JoVE Journal - Bioengineering
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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

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Cited by 11 •

2020

Electrowetting-based digital microfluidic is a technique that utilizes a voltage-driven change in the apparent contact angle of a microliter-volume droplet to facilitate its manipulation. Combining this with functionalized magnetic beads enables the integration of multiple laboratory unit operations for sample preparation and identification of pathogens using Enzyme-linked Immunosorbent Assay (ELISA).

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

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Cited by 8 •

2016

Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

Research

JoVE Journal - Biology
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Quantitation of Endothelial Cell Adhesiveness In Vitro

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Cited by 10 •

2015

We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.

Research

JoVE Journal - Environment

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

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Cited by 2 •

2018

Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.

Research

JoVE Journal - Biology
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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

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Cited by 15 •

2017

A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.

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