Negative Dielectrophoresis
Negative Dielectrophoresis - Related Videos
10:38
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Cited by 19 •
2013
Contactless dielectrophoresis (cDEP) achieves sorting and enrichment of particles via their intrinsic dielectric properties. Fluidic electrode channels replace metallic electrodes traditional to DEP, suiting cDEP to non-damaging sterile characterization and sorting of biological particles. We demonstrate how to prepare a cDEP microdevice and conduct cell characterization and sorting experiments.
01:23
Negative Regulator Molecules
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2019
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria. Three of the best-understood negative regulators are p53, p21, and retinoblastoma protein (Rb). The regulatory roles of each of these proteins were discovered after faulty copies were found in cells with uncontrolled replication (i.e., cancer). These...
01:23
Negative Externalities
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2025
A negative externality occurs when an economic transaction imposes unintended costs on third parties who are not directly involved in the market transaction. These external costs are not captured in the market price. Negative Externalities and Market Failure Consider a chemical manufacturing plant that produces industrial chemicals for sale. The plant's private costs include raw materials, labor, equipment, and maintenance, which are reflected in the market price of the chemicals. However,...
00:58
Positive, Negative, and Zero Work
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2025
Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...
06:56
Isolation of Double Negative αβ T Cells from the Kidney
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Cited by 26 •
2014
DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.
FAQs
These variables alter the effective polarizability of both the particle and surrounding medium, which determines how strongly the particle responds to the nonuniform field. Particle properties also contribute to this balance. Adjusting frequency, conductivity, or permittivity can therefore change particle motion and improve control over positioning, concentration, or separation in microscale systems.
The particle and its surrounding medium respond differently to an applied electric field. Their relative effective polarizabilities determine whether the induced dipole experiences a force toward or away from stronger-field regions. In negative dielectrophoresis, this contrast produces motion toward weaker-field areas, allowing engineers to control microscale objects without direct mechanical contact.
Particles with different electrical properties can respond differently under the same field conditions. Because the response depends on particle characteristics as well as medium conductivity, permittivity, and frequency, engineers can use these differences to influence positioning or separation. This principle supports the sorting of cells, beads, and other microscale objects in engineered fluidic environments.
A typical workflow establishes a nonuniform electric field within the microscale device, introduces the particles or cells, and adjusts relevant electrical and material conditions to produce the desired motion. The system can then guide objects toward lower-field regions for positioning, concentration, or separation. This contact-free approach is suited to controlled sample handling.
Engineers can apply the technique when they need to manipulate cells, beads, or other microscale objects without physical contact. Its ability to concentrate, position, and separate particles makes it useful in sample preparation and sorting workflows. These functions are especially relevant to microfluidic and lab-on-a-chip systems, where controlled handling is central to device operation.
The method provides a way to control object location through adjustable electrical conditions rather than direct mechanical handling. Within microfluidic and lab-on-a-chip platforms, that capability can support concentration, positioning, and separation functions. It also contributes to the development of electrically controlled microscale devices by integrating particle manipulation into compact engineering systems.